Siegert, SandraIST Austria ; Scherf, Brigitte G ; Del Punta, Karina ; Didkovsky, Nick ; Heintz, Nathaniel M ; Roska, Botond M
The mammalian brain is assembled from thousands of neuronal cell types that are organized in distinct circuits to perform behaviorally relevant computations. Transgenic mouse lines with selectively marked cell types would facilitate our ability to dissect functional components of complex circuits. We carried out a screen for cell type-specific green fluorescent protein expression in the retina using BAC transgenic mice from the GENSAT project. Among others, we identified mouse lines in which the inhibitory cell types of the night vision and directional selective circuit were selectively labeled. We quantified the stratification patterns to predict potential synaptic connectivity between marked cells of different lines and found that some of the lines enabled targeted recordings and imaging of cell types from developing or mature retinal circuits. Our results suggest the potential use of a stratification-based screening approach for characterizing neuronal circuitry in other layered brain structures, such as the neocortex.
This study was supported by Friedrich Miescher Institute funds, a US Office of Naval Research Naval International Cooperative Opportunities in Science and Technology Program grant, a Marie Curie Excellence grant, a National Center for Competence in Research in Genetics grant and a European Union HEALTH-F2-223156 grant to B.R., and by National Institute of Neurological Disorders and Stroke contracts N01NS02331 and HHSN271200723701C to N.H.
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Siegert S, Scherf B, Del Punta K, Didkovsky N, Heintz N, Roska B. Genetic address book for retinal cell types. Nature Neuroscience. 2009;12(9):1197-1204. doi:10.1038/nn.2370
Siegert, S., Scherf, B., Del Punta, K., Didkovsky, N., Heintz, N., & Roska, B. (2009). Genetic address book for retinal cell types. Nature Neuroscience, 12(9), 1197–1204. https://doi.org/10.1038/nn.2370
Siegert, Sandra, Brigitte Scherf, Karina Del Punta, Nick Didkovsky, Nathaniel Heintz, and Botond Roska. “Genetic Address Book for Retinal Cell Types.” Nature Neuroscience 12, no. 9 (2009): 1197–1204. https://doi.org/10.1038/nn.2370.
S. Siegert, B. Scherf, K. Del Punta, N. Didkovsky, N. Heintz, and B. Roska, “Genetic address book for retinal cell types,” Nature Neuroscience, vol. 12, no. 9, pp. 1197–1204, 2009.
Siegert S, Scherf B, Del Punta K, Didkovsky N, Heintz N, Roska B. 2009. Genetic address book for retinal cell types. Nature Neuroscience. 12(9), 1197–1204.
Siegert, Sandra, et al. “Genetic Address Book for Retinal Cell Types.” Nature Neuroscience, vol. 12, no. 9, Nature Publishing Group, 2009, pp. 1197–204, doi:10.1038/nn.2370.