Three-dimensional structure of respiratory complex I from Escherichia coli in ice in the presence of nucleotides

D. Morgan, L.A. Sazanov, Biochimica et Biophysica Acta - Bioenergetics 1777 (2008) 711–718.

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Abstract
Complex I (NADH:ubiquinone oxidoreductase) is the largest protein complex of bacterial and mitochondrial respiratory chains. The first three-dimensional structure of bacterial complex I in vitrified ice was determined by electron cryo-microscopy and single particle analysis. The structure of the Escherichia coli enzyme incubated with either NAD+ (as a reference) or NADH was calculated to 35 and 39 Å resolution, respectively. The X-ray structure of the peripheral arm of Thermus thermophilus complex I was docked into the reference EM structure. The model obtained indicates that Fe-S cluster N2 is close to the membrane domain interface, allowing for effective electron transfer to membrane-embedded quinone. At the current resolution, the structures in the presence of NAD+ or NADH are similar. Additionally, side-view class averages were calculated for the negatively stained bovine enzyme. The structures of bovine complex I in the presence of either NAD+ or NADH also appeared to be similar. These observations indicate that conformational changes upon reduction with NADH, suggested to occur by a range of studies, are smaller than had been thought previously. The model of the entire bacterial complex I could be built from the crystal structures of subcomplexes using the EM envelope described here.
Publishing Year
Date Published
2008-07-01
Journal Title
Biochimica et Biophysica Acta - Bioenergetics
Acknowledgement
This work was supported by the Medical Research Council.
Volume
1777
Issue
7-8
Page
711 - 718
IST-REx-ID

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Morgan D, Sazanov LA. Three-dimensional structure of respiratory complex I from Escherichia coli in ice in the presence of nucleotides. Biochimica et Biophysica Acta - Bioenergetics. 2008;1777(7-8):711-718. doi:10.1016/j.bbabio.2008.03.023
Morgan, D., & Sazanov, L. A. (2008). Three-dimensional structure of respiratory complex I from Escherichia coli in ice in the presence of nucleotides. Biochimica et Biophysica Acta - Bioenergetics, 1777(7–8), 711–718. https://doi.org/10.1016/j.bbabio.2008.03.023
Morgan, David, and Leonid A Sazanov. “Three-Dimensional Structure of Respiratory Complex I from Escherichia Coli in Ice in the Presence of Nucleotides.” Biochimica et Biophysica Acta - Bioenergetics 1777, no. 7–8 (2008): 711–18. https://doi.org/10.1016/j.bbabio.2008.03.023.
D. Morgan and L. A. Sazanov, “Three-dimensional structure of respiratory complex I from Escherichia coli in ice in the presence of nucleotides,” Biochimica et Biophysica Acta - Bioenergetics, vol. 1777, no. 7–8, pp. 711–718, 2008.
Morgan D, Sazanov LA. 2008. Three-dimensional structure of respiratory complex I from Escherichia coli in ice in the presence of nucleotides. Biochimica et Biophysica Acta - Bioenergetics. 1777(7–8), 711–718.
Morgan, David, and Leonid A. Sazanov. “Three-Dimensional Structure of Respiratory Complex I from Escherichia Coli in Ice in the Presence of Nucleotides.” Biochimica et Biophysica Acta - Bioenergetics, vol. 1777, no. 7–8, Elsevier, 2008, pp. 711–18, doi:10.1016/j.bbabio.2008.03.023.

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