Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme

C. Yip, M. Harbour, K. Jayawardena, I. Fearnley, L.A. Sazanov, Journal of Biological Chemistry 286 (2011) 5023–5033.

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Abstract
Modern α-proteobacteria are thought to be closely related to the ancient symbiont of eukaryotes, an ancestor of mitochondria. Respiratory complex I from α-proteobacteria and mitochondria is well conserved at the level of the 14 "core" subunits, consistent with that notion. Mitochondrial complex I contains the core subunits, present in all species, and up to 31 "supernumerary" subunits, generally thought to have originated only within eukaryotic lineages. However, the full protein composition of an α-proteobacterial complex I has not been established previously. Here, we report the first purification and characterization of complex I from the α-proteobacterium Paracoccus denitrificans. Single particle electron microscopy shows that the complex has a well defined L-shape. Unexpectedly, in addition to the 14 core subunits, the enzyme also contains homologues of three supernumerary mitochondrial subunits as follows: B17.2, AQDQ/18, and 13 kDa (bovine nomenclature). This finding suggests that evolution of complex I via addition of supernumerary or "accessory" subunits started before the original endosymbiotic event that led to the creation of the eukaryotic cell. It also provides further confirmation that α-proteobacteria are the closest extant relatives of mitochondria.
Publishing Year
Date Published
2011-02-18
Journal Title
Journal of Biological Chemistry
Acknowledgement
This work was supported by the Medical Research Council.
Volume
286
Issue
7
Page
5023 - 5033
IST-REx-ID

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Yip C, Harbour M, Jayawardena K, Fearnley I, Sazanov LA. Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme. Journal of Biological Chemistry. 2011;286(7):5023-5033. doi:10.1074/jbc.M110.194993
Yip, C., Harbour, M., Jayawardena, K., Fearnley, I., & Sazanov, L. A. (2011). Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme. Journal of Biological Chemistry, 286(7), 5023–5033. https://doi.org/10.1074/jbc.M110.194993
Yip, Chui, Michael Harbour, Kamburapola Jayawardena, Ian Fearnley, and Leonid A Sazanov. “Evolution of Respiratory Complex I "Supernumerary" Subunits Are Present in the α-Proteobacterial Enzyme.” Journal of Biological Chemistry 286, no. 7 (2011): 5023–33. https://doi.org/10.1074/jbc.M110.194993.
C. Yip, M. Harbour, K. Jayawardena, I. Fearnley, and L. A. Sazanov, “Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme,” Journal of Biological Chemistry, vol. 286, no. 7, pp. 5023–5033, 2011.
Yip C, Harbour M, Jayawardena K, Fearnley I, Sazanov LA. 2011. Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme. Journal of Biological Chemistry. 286(7), 5023–5033.
Yip, Chui, et al. “Evolution of Respiratory Complex I "Supernumerary" Subunits Are Present in the α-Proteobacterial Enzyme.” Journal of Biological Chemistry, vol. 286, no. 7, American Society for Biochemistry and Molecular Biology, 2011, pp. 5023–33, doi:10.1074/jbc.M110.194993.

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