The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase

Y. Tone, H. Inoue, S. Hara, C. Yokoyama, T. Hatae, H. Oida, S. Narumiya, R. Shigemoto, S. Yukawa, T. Tanabe, European Journal of Cell Biology 72 (1997) 268–277.

Download
No fulltext has been uploaded. References only!
Journal Article | Published
Author
; ; ; ; ; ; ; ; ;
Abstract
The cloned cDNA for rat prostacyclin synthase was found to contain a 1503-bp open reading frame which encoded a 501-amino acid protein sharing 84% identity with the human enzyme. RNA blot analysis revealed that the rat prostacyclin synthase mRNA, as a single species of 2.1 kb, is expressed abundantly in the aorta and uterus. High levels of expression were also observed in the stomach, lung, heart, testis, liver, and skeletal muscle. Low but significant expression was also seen in the brain and kidney. Furthermore, the regional distribution and cellular localization of prostacyclin synthase mRNA were examined by in situ hybridization analysis of rat tissue sections. The definitive signals for the mRNA were localized in smooth muscle cells of the arteries, bronchi and uterus, and in the cells of the fibrous tunic surrounding the seminiferous tubules, which are characterized as smooth muscle cells. Besides smooth muscle cells, signal were also detected in the fibroblasts of the heart myocardium, lung parenchyma cells and kidney inner medulla tubules and interstitial cells.
Publishing Year
Date Published
1997-03-01
Journal Title
European Journal of Cell Biology
Volume
72
Issue
3
Page
268 - 277
IST-REx-ID

Cite this

Tone Y, Inoue H, Hara S, et al. The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase. European Journal of Cell Biology. 1997;72(3):268-277.
Tone, Y., Inoue, H., Hara, S., Yokoyama, C., Hatae, T., Oida, H., … Tanabe, T. (1997). The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase. European Journal of Cell Biology, 72(3), 268–277.
Tone, Yoshinori, Hiroyasu Inoue, Shuntaro Hara, Chieko Yokoyama, Toshihisa Hatae, Hiroji Oida, Shuh Narumiya, Ryuichi Shigemoto, Susumu Yukawa, and Tadashi Tanabe. “The Regional Distribution and Cellular Localization of MRNA Encoding Rat Prostacyclin Synthase.” European Journal of Cell Biology 72, no. 3 (1997): 268–77.
Y. Tone et al., “The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase,” European Journal of Cell Biology, vol. 72, no. 3, pp. 268–277, 1997.
Tone Y, Inoue H, Hara S, Yokoyama C, Hatae T, Oida H, Narumiya S, Shigemoto R, Yukawa S, Tanabe T. 1997. The regional distribution and cellular localization of mRNA encoding rat prostacyclin synthase. European Journal of Cell Biology. 72(3), 268–277.
Tone, Yoshinori, et al. “The Regional Distribution and Cellular Localization of MRNA Encoding Rat Prostacyclin Synthase.” European Journal of Cell Biology, vol. 72, no. 3, Elsevier, 1997, pp. 268–77.

Export

Marked Publications

Open Data IST Research Explorer

Search this title in

Google Scholar