Subtype-specific expression of Group III metabotropic glutamate receptors and Ca2+ channels in single nerve terminals

C. Millán, R. Luján, R. Shigemoto, J. Sánchez Prieto, Journal of Biological Chemistry 277 (2002) 47796–47803.

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Abstract
The release properties of glutamatergic nerve terminals are influenced by a number of factors, including the subtype of voltage-dependent calcium channel and the presence of presynaptic autoreceptors. Group III metabotropic glutamate receptors (mGluRs) mediate feedback inhibition of glutamate release by inhibiting Ca2+ channel activity. By imaging Ca2+ in preparations of cerebrocortical nerve terminals, we show that voltage-dependent Ca2+ channels are distributed in a heterogeneous manner in individual nerve terminals. Presynaptic terminals contained only N-type (47.5%; conotoxin GVIA-sensitive), P/Q-type (3.9%; agatoxin IVA-sensitive), or both N- and P/Q-type (42.6%) Ca2+ channels, although the remainder of the terminals (6.1%) were insensitive to these two toxins. In this preparation, two mGluRs with high and low affinity for L(+)-2-amino-4-phosphonobutyrate were identified by immunocytochemistry as mGluR4 and mGluR7, respectively. These receptors were responsible for 22.2 and 24.1% reduction of glutamate release, and they reduced the Ca2+ response in 24.4 and 30.3% of the nerve terminals, respectively. Interestingly, mGluR4 was largely (73.7%) located in nerve terminals expressing both N- and P/Q-type Ca2+ channels, whereas mGluR7 was predominantly (69.9%) located in N-type Ca2+ channel-expressing terminals. This specific coexpression of different group III mGluRs and Ca2+ channels may endow synaptic terminals with distinct release properties and reveals the existence of a high degree of presynaptic heterogeneity.
Publishing Year
Date Published
2002-12-02
Journal Title
Journal of Biological Chemistry
Volume
277
Issue
49
Page
47796 - 47803
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Millán C, Luján R, Shigemoto R, Sánchez Prieto J. Subtype-specific expression of Group III metabotropic glutamate receptors and Ca2+ channels in single nerve terminals. Journal of Biological Chemistry. 2002;277(49):47796-47803. doi:10.1074/jbc.M207531200
Millán, C., Luján, R., Shigemoto, R., & Sánchez Prieto, J. (2002). Subtype-specific expression of Group III metabotropic glutamate receptors and Ca2+ channels in single nerve terminals. Journal of Biological Chemistry, 277(49), 47796–47803. https://doi.org/10.1074/jbc.M207531200
Millán, Carmelo, Rafael Luján, Ryuichi Shigemoto, and José Sánchez Prieto. “Subtype-Specific Expression of Group III Metabotropic Glutamate Receptors and Ca2+ Channels in Single Nerve Terminals.” Journal of Biological Chemistry 277, no. 49 (2002): 47796–803. https://doi.org/10.1074/jbc.M207531200.
C. Millán, R. Luján, R. Shigemoto, and J. Sánchez Prieto, “Subtype-specific expression of Group III metabotropic glutamate receptors and Ca2+ channels in single nerve terminals,” Journal of Biological Chemistry, vol. 277, no. 49, pp. 47796–47803, 2002.
Millán C, Luján R, Shigemoto R, Sánchez Prieto J. 2002. Subtype-specific expression of Group III metabotropic glutamate receptors and Ca2+ channels in single nerve terminals. Journal of Biological Chemistry. 277(49), 47796–47803.
Millán, Carmelo, et al. “Subtype-Specific Expression of Group III Metabotropic Glutamate Receptors and Ca2+ Channels in Single Nerve Terminals.” Journal of Biological Chemistry, vol. 277, no. 49, American Society for Biochemistry and Molecular Biology, 2002, pp. 47796–803, doi:10.1074/jbc.M207531200.

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