Developmental refinement of vesicle cycling at Schaffer collateral synapses

T. Rose, P. Schönenberger, K. Jezek, T. Oertner, Neuron 77 (2013) 1109–1121.

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Journal Article | Published | English
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Abstract
At synapses formed between dissociated neurons, about half of all synaptic vesicles are refractory to evoked release, forming the so-called "resting pool." Here, we use optical measurements of vesicular pH to study developmental changes in pool partitioning and vesicle cycling in cultured hippocampal slices. Two-photon imaging of a genetically encoded two-color release sensor (ratio-sypHy) allowed us to perform calibrated measurements at individual Schaffer collateral boutons. Mature boutons released a large fraction of their vesicles during simulated place field activity, and vesicle retrieval rates were 7-fold higher compared to immature boutons. Saturating stimulation mobilized essentially all vesicles at mature synapses. Resting pool formation and a concomitant reduction in evoked release was induced by chronic depolarization but not by acute inhibition of the protein phosphatase calcineurin. We conclude that synapses in CA1 undergo a prominent refinement of vesicle use during early postnatal development that is not recapitulated in dissociated neuronal culture.
Publishing Year
Date Published
2013-03-20
Journal Title
Neuron
Volume
77
Issue
6
Page
1109 - 1121
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Rose T, Schönenberger P, Jezek K, Oertner T. Developmental refinement of vesicle cycling at Schaffer collateral synapses. Neuron. 2013;77(6):1109-1121. doi:10.1016/j.neuron.2013.01.021
Rose, T., Schönenberger, P., Jezek, K., & Oertner, T. (2013). Developmental refinement of vesicle cycling at Schaffer collateral synapses. Neuron, 77(6), 1109–1121. https://doi.org/10.1016/j.neuron.2013.01.021
Rose, Tobias, Philipp Schönenberger, Karel Jezek, and Thomas Oertner. “Developmental Refinement of Vesicle Cycling at Schaffer Collateral Synapses.” Neuron 77, no. 6 (2013): 1109–21. https://doi.org/10.1016/j.neuron.2013.01.021.
T. Rose, P. Schönenberger, K. Jezek, and T. Oertner, “Developmental refinement of vesicle cycling at Schaffer collateral synapses,” Neuron, vol. 77, no. 6, pp. 1109–1121, 2013.
Rose T, Schönenberger P, Jezek K, Oertner T. 2013. Developmental refinement of vesicle cycling at Schaffer collateral synapses. Neuron. 77(6), 1109–1121.
Rose, Tobias, et al. “Developmental Refinement of Vesicle Cycling at Schaffer Collateral Synapses.” Neuron, vol. 77, no. 6, Elsevier, 2013, pp. 1109–21, doi:10.1016/j.neuron.2013.01.021.

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