{"publisher":"eLife Sciences Publications","intvolume":"        10","title":"Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism","publication_status":"published","date_updated":"2023-08-14T06:53:33Z","ec_funded":1,"isi":1,"quality_controlled":"1","abstract":[{"text":"The developmental strategies used by progenitor cells to endure a safe journey from their induction place towards the site of terminal differentiation are still poorly understood. Here we uncovered a progenitor cell allocation mechanism that stems from an incomplete process of epithelial delamination that allows progenitors to coordinate their movement with adjacent extra-embryonic tissues. Progenitors of the zebrafish laterality organ originate from the surface epithelial enveloping layer by an apical constriction process of cell delamination. During this process, progenitors retain long-term apical contacts that enable the epithelial layer to pull a subset of progenitors along their way towards the vegetal pole. The remaining delaminated progenitors follow apically-attached progenitors’ movement by a co-attraction mechanism, avoiding sequestration by the adjacent endoderm, ensuring their fate and collective allocation at the differentiation site. Thus, we reveal that incomplete delamination serves as a cellular platform for coordinated tissue movements during development. Impact Statement: Incomplete delamination serves as a cellular platform for coordinated tissue movements during development, guiding newly formed progenitor cell groups to the differentiation site.","lang":"eng"}],"language":[{"iso":"eng"}],"date_published":"2021-08-27T00:00:00Z","date_created":"2021-09-12T22:01:23Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","external_id":{"isi":["000700428500001"],"pmid":["34448451"]},"year":"2021","oa_version":"Published Version","pmid":1,"month":"08","publication_identifier":{"eissn":["2050-084X"]},"article_type":"original","doi":"10.7554/eLife.66483","day":"27","author":[{"last_name":"Pulgar","first_name":"Eduardo","full_name":"Pulgar, Eduardo"},{"full_name":"Schwayer, Cornelia","first_name":"Cornelia","id":"3436488C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5130-2226","last_name":"Schwayer"},{"last_name":"Guerrero","full_name":"Guerrero, Néstor","first_name":"Néstor"},{"full_name":"López, Loreto","first_name":"Loreto","last_name":"López"},{"first_name":"Susana","full_name":"Márquez, Susana","last_name":"Márquez"},{"full_name":"Härtel, Steffen","first_name":"Steffen","last_name":"Härtel"},{"last_name":"Soto","first_name":"Rodrigo","full_name":"Soto, Rodrigo"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl Philipp","first_name":"Carl Philipp"},{"first_name":"Miguel L.","full_name":"Concha, Miguel L.","last_name":"Concha"}],"article_processing_charge":"Yes","file_date_updated":"2022-05-13T08:03:37Z","keyword":["cell delamination","apical constriction","dragging","mechanical forces","collective 18 locomotion","dorsal forerunner cells","zebrafish"],"_id":"9999","oa":1,"file":[{"file_size":9010446,"content_type":"application/pdf","checksum":"a3f82b0499cc822ac1eab48a01f3f57e","date_created":"2022-05-13T08:03:37Z","file_id":"11371","success":1,"access_level":"open_access","file_name":"2021_eLife_Pulgar.pdf","date_updated":"2022-05-13T08:03:37Z","relation":"main_file","creator":"dernst"}],"scopus_import":"1","volume":10,"project":[{"call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573"}],"publication":"eLife","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"citation":{"apa":"Pulgar, E., Schwayer, C., Guerrero, N., López, L., Márquez, S., Härtel, S., … Concha, M. L. (2021). Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.66483\">https://doi.org/10.7554/eLife.66483</a>","chicago":"Pulgar, Eduardo, Cornelia Schwayer, Néstor Guerrero, Loreto López, Susana Márquez, Steffen Härtel, Rodrigo Soto, Carl Philipp Heisenberg, and Miguel L. Concha. “Apical Contacts Stemming from Incomplete Delamination Guide Progenitor Cell Allocation through a Dragging Mechanism.” <i>ELife</i>. eLife Sciences Publications, 2021. <a href=\"https://doi.org/10.7554/eLife.66483\">https://doi.org/10.7554/eLife.66483</a>.","short":"E. Pulgar, C. Schwayer, N. Guerrero, L. López, S. Márquez, S. Härtel, R. Soto, C.P. Heisenberg, M.L. Concha, ELife 10 (2021).","mla":"Pulgar, Eduardo, et al. “Apical Contacts Stemming from Incomplete Delamination Guide Progenitor Cell Allocation through a Dragging Mechanism.” <i>ELife</i>, vol. 10, e66483, eLife Sciences Publications, 2021, doi:<a href=\"https://doi.org/10.7554/eLife.66483\">10.7554/eLife.66483</a>.","ieee":"E. Pulgar <i>et al.</i>, “Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism,” <i>eLife</i>, vol. 10. eLife Sciences Publications, 2021.","ama":"Pulgar E, Schwayer C, Guerrero N, et al. Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. <i>eLife</i>. 2021;10. doi:<a href=\"https://doi.org/10.7554/eLife.66483\">10.7554/eLife.66483</a>","ista":"Pulgar E, Schwayer C, Guerrero N, López L, Márquez S, Härtel S, Soto R, Heisenberg CP, Concha ML. 2021. Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism. eLife. 10, e66483."},"status":"public","department":[{"_id":"CaHe"}],"type":"journal_article","ddc":["570"],"article_number":"e66483","has_accepted_license":"1"}