--- res: bibo_abstract: - Mosaic analysis with double markers (MADM) offers one approach to visualize and concomitantly manipulate genetically defined cells in mice with single-cell resolution. MADM applications include the analysis of lineage, single-cell morphology and physiology, genomic imprinting phenotypes, and dissection of cell-autonomous gene functions in vivo in health and disease. Yet, MADM can only be applied to <25% of all mouse genes on select chromosomes to date. To overcome this limitation, we generate transgenic mice with knocked-in MADM cassettes near the centromeres of all 19 autosomes and validate their use across organs. With this resource, >96% of the entire mouse genome can now be subjected to single-cell genetic mosaic analysis. Beyond a proof of principle, we apply our MADM library to systematically trace sister chromatid segregation in distinct mitotic cell lineages. We find striking chromosome-specific biases in segregation patterns, reflecting a putative mechanism for the asymmetric segregation of genetic determinants in somatic stem cell division.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Ximena foaf_name: Contreras, Ximena foaf_surname: Contreras foaf_workInfoHomepage: http://www.librecat.org/personId=475990FE-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Nicole foaf_name: Amberg, Nicole foaf_surname: Amberg foaf_workInfoHomepage: http://www.librecat.org/personId=4CD6AAC6-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-3183-8207 - foaf_Person: foaf_givenName: Amarbayasgalan foaf_name: Davaatseren, Amarbayasgalan foaf_surname: Davaatseren foaf_workInfoHomepage: http://www.librecat.org/personId=70ADC922-B424-11E9-99E3-BA18E6697425 - foaf_Person: foaf_givenName: Andi H foaf_name: Hansen, Andi H foaf_surname: Hansen foaf_workInfoHomepage: http://www.librecat.org/personId=38853E16-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Johanna foaf_name: Sonntag, Johanna foaf_surname: Sonntag foaf_workInfoHomepage: http://www.librecat.org/personId=32FE7D7C-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Lill foaf_name: Andersen, Lill foaf_surname: Andersen - foaf_Person: foaf_givenName: Tina foaf_name: Bernthaler, Tina foaf_surname: Bernthaler - foaf_Person: foaf_givenName: Carmen foaf_name: Streicher, Carmen foaf_surname: Streicher foaf_workInfoHomepage: http://www.librecat.org/personId=36BCB99C-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Anna-Magdalena foaf_name: Heger, Anna-Magdalena foaf_surname: Heger foaf_workInfoHomepage: http://www.librecat.org/personId=4B76FFD2-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Randy L. foaf_name: Johnson, Randy L. foaf_surname: Johnson - foaf_Person: foaf_givenName: Lindsay A. foaf_name: Schwarz, Lindsay A. foaf_surname: Schwarz - foaf_Person: foaf_givenName: Liqun foaf_name: Luo, Liqun foaf_surname: Luo - foaf_Person: foaf_givenName: Thomas foaf_name: Rülicke, Thomas foaf_surname: Rülicke - foaf_Person: foaf_givenName: Simon foaf_name: Hippenmeyer, Simon foaf_surname: Hippenmeyer foaf_workInfoHomepage: http://www.librecat.org/personId=37B36620-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-2279-1061 bibo_doi: 10.1016/j.celrep.2021.109274 bibo_issue: '12' bibo_volume: 35 dct_date: 2021^xs_gYear dct_identifier: - UT:000664463600016 dct_isPartOf: - http://id.crossref.org/issn/22111247 dct_language: eng dct_publisher: Cell Press@ dct_title: A genome-wide library of MADM mice for single-cell genetic mosaic analysis@ ...