Genome-wide demethylation of Arabidopsis endosperm

Hsieh T-F, Ibarra CA, Silva P, Zemach A, Eshed-Williams L, Fischer RL, ZILBERMAN D. 2009. Genome-wide demethylation of Arabidopsis endosperm. Science. 324(5933), 1451–1454.


Journal Article | Published | English

Scopus indexed
Author
Hsieh, Tzung-Fu; Ibarra, Christian A.; Silva, Pedro; Zemach, Assaf; Eshed-Williams, Leor; Fischer, Robert L.; ZILBERMAN, DanielIST Austria
Abstract
Parent-of-origin-specific (imprinted) gene expression is regulated in Arabidopsis thaliana endosperm by cytosine demethylation of the maternal genome mediated by the DNA glycosylase DEMETER, but the extent of the methylation changes is not known. Here, we show that virtually the entire endosperm genome is demethylated, coupled with extensive local non-CG hypermethylation of small interfering RNA–targeted sequences. Mutation of DEMETER partially restores endosperm CG methylation to levels found in other tissues, indicating that CG demethylation is specific to maternal sequences. Endosperm demethylation is accompanied by CHH hypermethylation of embryo transposable elements. Our findings demonstrate extensive reconfiguration of the endosperm methylation landscape that likely reinforces transposon silencing in the embryo.
Publishing Year
Date Published
2009-06-12
Journal Title
Science
Volume
324
Issue
5933
Page
1451-1454
ISSN
eISSN
IST-REx-ID

Cite this

Hsieh T-F, Ibarra CA, Silva P, et al. Genome-wide demethylation of Arabidopsis endosperm. Science. 2009;324(5933):1451-1454. doi:10.1126/science.1172417
Hsieh, T.-F., Ibarra, C. A., Silva, P., Zemach, A., Eshed-Williams, L., Fischer, R. L., & ZILBERMAN, D. (2009). Genome-wide demethylation of Arabidopsis endosperm. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1172417
Hsieh, Tzung-Fu, Christian A. Ibarra, Pedro Silva, Assaf Zemach, Leor Eshed-Williams, Robert L. Fischer, and Daniel ZILBERMAN. “Genome-Wide Demethylation of Arabidopsis Endosperm.” Science. American Association for the Advancement of Science, 2009. https://doi.org/10.1126/science.1172417.
T.-F. Hsieh et al., “Genome-wide demethylation of Arabidopsis endosperm,” Science, vol. 324, no. 5933. American Association for the Advancement of Science, pp. 1451–1454, 2009.
Hsieh T-F, Ibarra CA, Silva P, Zemach A, Eshed-Williams L, Fischer RL, ZILBERMAN D. 2009. Genome-wide demethylation of Arabidopsis endosperm. Science. 324(5933), 1451–1454.
Hsieh, Tzung-Fu, et al. “Genome-Wide Demethylation of Arabidopsis Endosperm.” Science, vol. 324, no. 5933, American Association for the Advancement of Science, 2009, pp. 1451–54, doi:10.1126/science.1172417.
All files available under the following license(s):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]

Link(s) to Main File(s)
Access Level
OA Open Access

Export

Marked Publications

Open Data IST Research Explorer

Sources

PMID: 19520962
PubMed | Europe PMC

Search this title in

Google Scholar