{"publication_status":"published","citation":{"ieee":"D. B. Lyons and D. Zilberman, “DDM1 and Lsh remodelers allow methylation of DNA wrapped in nucleosomes,” <i>eLife</i>, vol. 6. eLife Sciences Publications, 2017.","short":"D.B. Lyons, D. Zilberman, ELife 6 (2017).","apa":"Lyons, D. B., &#38; Zilberman, D. (2017). DDM1 and Lsh remodelers allow methylation of DNA wrapped in nucleosomes. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/elife.30674\">https://doi.org/10.7554/elife.30674</a>","chicago":"Lyons, David B, and Daniel Zilberman. “DDM1 and Lsh Remodelers Allow Methylation of DNA Wrapped in Nucleosomes.” <i>ELife</i>. eLife Sciences Publications, 2017. <a href=\"https://doi.org/10.7554/elife.30674\">https://doi.org/10.7554/elife.30674</a>.","ama":"Lyons DB, Zilberman D. DDM1 and Lsh remodelers allow methylation of DNA wrapped in nucleosomes. <i>eLife</i>. 2017;6. doi:<a href=\"https://doi.org/10.7554/elife.30674\">10.7554/elife.30674</a>","mla":"Lyons, David B., and Daniel Zilberman. “DDM1 and Lsh Remodelers Allow Methylation of DNA Wrapped in Nucleosomes.” <i>ELife</i>, vol. 6, e30674, eLife Sciences Publications, 2017, doi:<a href=\"https://doi.org/10.7554/elife.30674\">10.7554/elife.30674</a>.","ista":"Lyons DB, Zilberman D. 2017. DDM1 and Lsh remodelers allow methylation of DNA wrapped in nucleosomes. eLife. 6, e30674."},"month":"11","publication":"eLife","scopus_import":"1","volume":6,"extern":"1","ddc":["570"],"status":"public","date_updated":"2021-12-14T07:54:36Z","has_accepted_license":"1","author":[{"last_name":"Lyons","first_name":"David B","full_name":"Lyons, David B"},{"full_name":"Zilberman, Daniel","first_name":"Daniel","id":"6973db13-dd5f-11ea-814e-b3e5455e9ed1","last_name":"Zilberman","orcid":"0000-0002-0123-8649"}],"external_id":{"pmid":["29140247"]},"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","_id":"9445","year":"2017","quality_controlled":"1","doi":"10.7554/elife.30674","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"type":"journal_article","publisher":"eLife Sciences Publications","article_type":"original","article_number":"e30674","file":[{"file_id":"9446","access_level":"open_access","success":1,"date_created":"2021-06-02T14:33:36Z","file_size":1603102,"date_updated":"2021-06-02T14:33:36Z","checksum":"4cfcdd67511ae4aed3d993550e46e146","creator":"cziletti","file_name":"2017_eLife_Lyons.pdf","relation":"main_file","content_type":"application/pdf"}],"department":[{"_id":"DaZi"}],"date_published":"2017-11-15T00:00:00Z","oa_version":"Published Version","pmid":1,"abstract":[{"lang":"eng","text":"Cytosine methylation regulates essential genome functions across eukaryotes, but the fundamental question of whether nucleosomal or naked DNA is the preferred substrate of plant and animal methyltransferases remains unresolved. Here, we show that genetic inactivation of a single DDM1/Lsh family nucleosome remodeler biases methylation toward inter-nucleosomal linker DNA in Arabidopsis thaliana and mouse. We find that DDM1 enables methylation of DNA bound to the nucleosome, suggesting that nucleosome-free DNA is the preferred substrate of eukaryotic methyltransferases in vivo. Furthermore, we show that simultaneous mutation of DDM1 and linker histone H1 in Arabidopsis reproduces the strong linker-specific methylation patterns of species that diverged from flowering plants and animals over a billion years ago. Our results indicate that in the absence of remodeling, nucleosomes are strong barriers to DNA methyltransferases. Linker-specific methylation can evolve simply by breaking the connection between nucleosome remodeling and DNA methylation."}],"oa":1,"language":[{"iso":"eng"}],"date_created":"2021-06-02T14:28:58Z","publication_identifier":{"eissn":["2050-084X"]},"file_date_updated":"2021-06-02T14:33:36Z","title":"DDM1 and Lsh remodelers allow methylation of DNA wrapped in nucleosomes","article_processing_charge":"No","day":"15","intvolume":"         6"}