RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells

J. Sedzinski, E.B. Hannezo, F. Tu, M. Biro, J. Wallingford, Journal of Cell Science 130 (2017).

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Journal Article | Published | English
Author
Sedzinski, Jakub; Hannezo, Edouard IST Austria ; Tu, Fan; Biro, Maté; Wallingford, John
Abstract
Homeostatic replacement of epithelial cells from basal precursors is a multistep process involving progenitor cell specification, radial intercalation and, finally, apical surface emergence. Recent data demonstrate that actin-based pushing under the control of the formin protein Fmn1 drives apical emergence in nascent multiciliated epithelial cells (MCCs), but little else is known about this actin network or the control of Fmn1. Here, we explore the role of the small GTPase RhoA in MCC apical emergence. Disruption of RhoA function reduced the rate of apical surface expansion and decreased the final size of the apical domain. Analysis of cell shapes suggests that RhoA alters the balance of forces exerted on the MCC apical surface. Finally, quantitative time-lapse imaging and fluorescence recovery after photobleaching studies argue that RhoA works in concert with Fmn1 to control assembly of the specialized apical actin network in MCCs. These data provide new molecular insights into epithelial apical surface assembly and could also shed light on mechanisms of apical lumen formation
Publishing Year
Date Published
2017-01-01
Journal Title
Journal of Cell Science
Volume
130
Issue
5
IST-REx-ID
936

Cite this

Sedzinski J, Hannezo EB, Tu F, Biro M, Wallingford J. RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells . Journal of Cell Science. 2017;130(5). doi:10.1242/jcs.202234
Sedzinski, J., Hannezo, E. B., Tu, F., Biro, M., & Wallingford, J. (2017). RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells . Journal of Cell Science, 130(5). https://doi.org/10.1242/jcs.202234
Sedzinski, Jakub, Edouard B Hannezo, Fan Tu, Maté Biro, and John Wallingford. “RhoA Regulates Actin Network Dynamics during Apical Surface Emergence in Multiciliated Epithelial Cells .” Journal of Cell Science 130, no. 5 (2017). https://doi.org/10.1242/jcs.202234.
J. Sedzinski, E. B. Hannezo, F. Tu, M. Biro, and J. Wallingford, “RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells ,” Journal of Cell Science, vol. 130, no. 5, 2017.
Sedzinski J, Hannezo EB, Tu F, Biro M, Wallingford J. 2017. RhoA regulates actin network dynamics during apical surface emergence in multiciliated epithelial cells . Journal of Cell Science. 130(5).
Sedzinski, Jakub, et al. “RhoA Regulates Actin Network Dynamics during Apical Surface Emergence in Multiciliated Epithelial Cells .” Journal of Cell Science, vol. 130, no. 5, Company of Biologists, 2017, doi:10.1242/jcs.202234.

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