IST Austria Thesis
Metabolic adaptation is a critical feature of migrating cells. It tunes the metabolic programs of migrating cells to allow them to efficiently exert their crucial roles in development, inflammatory responses and tumor metastasis. Cell migration through physically challenging contexts requires energy. However, how the metabolic reprogramming that underlies in vivo cell invasion is controlled is still unanswered. In my PhD project, I identify a novel conserved metabolic shift in Drosophila melanogaster immune cells that by modulating their bioenergetic potential controls developmentally programmed tissue invasion. We show that this regulation requires a novel conserved nuclear protein, named Atossa. Atossa enhances the transcription of a set of proteins, including an RNA helicase Porthos and two metabolic enzymes, each of which increases the tissue invasion of leading Drosophila macrophages and can rescue the atossa mutant phenotype. Porthos selectively regulates the translational efficiency of a subset of mRNAs containing a 5’-UTR cis-regulatory TOP-like sequence. These 5’TOPL mRNA targets encode mitochondrial-related proteins, including subunits of mitochondrial oxidative phosphorylation (OXPHOS) components III and V and other metabolic-related proteins. Porthos powers up mitochondrial OXPHOS to engender a sufficient ATP supply, which is required for tissue invasion of leading macrophages. Atossa’s two vertebrate orthologs rescue the invasion defect. In my PhD project, I elucidate that Atossa displays a conserved developmental metabolic control to modulate metabolic capacities and the cellular energy state, through altered transcription and translation, to aid the tissue infiltration of leading cells into energy demanding barriers.
Also, I would like to express my appreciation and thanks to the Bioimaging facility, LSF, GSO, library, and IT people at IST Austria.
Emtenani S. Metabolic regulation of Drosophila macrophage tissue invasion. 2020. doi:10.15479/AT:ISTA:8983
Emtenani, S. (2020). Metabolic regulation of Drosophila macrophage tissue invasion. IST Austria. https://doi.org/10.15479/AT:ISTA:8983
Emtenani, Shamsi. “Metabolic Regulation of Drosophila Macrophage Tissue Invasion.” IST Austria, 2020. https://doi.org/10.15479/AT:ISTA:8983.
S. Emtenani, “Metabolic regulation of Drosophila macrophage tissue invasion,” IST Austria, 2020.
Emtenani S. 2020. Metabolic regulation of Drosophila macrophage tissue invasion. IST Austria.
Emtenani, Shamsi. Metabolic Regulation of Drosophila Macrophage Tissue Invasion. IST Austria, 2020, doi:10.15479/AT:ISTA:8983.
Thesis_Shamsi_Emtenani_pdfA.pdf 10.85 MB
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Part of this Dissertation
Part of this Dissertation