--- res: bibo_abstract: - The actin-related protein (Arp)2/3 complex nucleates branched actin filament networks pivotal for cell migration, endocytosis and pathogen infection. Its activation is tightly regulated and involves complex structural rearrangements and actin filament binding, which are yet to be understood. Here, we report a 9.0 Å resolution structure of the actin filament Arp2/3 complex branch junction in cells using cryo-electron tomography and subtomogram averaging. This allows us to generate an accurate model of the active Arp2/3 complex in the branch junction and its interaction with actin filaments. Notably, our model reveals a previously undescribed set of interactions of the Arp2/3 complex with the mother filament, significantly different to the previous branch junction model. Our structure also indicates a central role for the ArpC3 subunit in stabilizing the active conformation.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Florian foaf_name: Fäßler, Florian foaf_surname: Fäßler foaf_workInfoHomepage: http://www.librecat.org/personId=404F5528-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-7149-769X - foaf_Person: foaf_givenName: Georgi A foaf_name: Dimchev, Georgi A foaf_surname: Dimchev foaf_workInfoHomepage: http://www.librecat.org/personId=38C393BE-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8370-6161 - foaf_Person: foaf_givenName: Victor-Valentin foaf_name: Hodirnau, Victor-Valentin foaf_surname: Hodirnau foaf_workInfoHomepage: http://www.librecat.org/personId=3661B498-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: William foaf_name: Wan, William foaf_surname: Wan - foaf_Person: foaf_givenName: Florian KM foaf_name: Schur, Florian KM foaf_surname: Schur foaf_workInfoHomepage: http://www.librecat.org/personId=48AD8942-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-4790-8078 bibo_doi: 10.1038/s41467-020-20286-x bibo_volume: 11 dct_date: 2020^xs_gYear dct_identifier: - UT:000603078000003 dct_isPartOf: - http://id.crossref.org/issn/2041-1723 dct_language: eng dct_publisher: Springer Nature@ dct_subject: - General Biochemistry - Genetics and Molecular Biology - General Physics and Astronomy - General Chemistry dct_title: Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction@ ...