Perelygin, Andrey A ; Kondrashov, FyodorIST Austria ; Rogozin, Igor B ; Brinton, Margo A
The polymeric ubiquitin (poly-u) genes are composed of tandem 228-bp repeats with no spacer sequences between individual monomer units. Ubiquitin is one of the most conserved proteins known to date, and the individual units within a number of poly-u genes are significantly more similar to each other than would be expected if each unit evolved independently. It has been proposed that the rather striking similarity among poly-u monomers in some lineages is caused by a series of homogenization events. Here we report the sequences of the polyubiquitin-C (Ubc) genes in two mouse strains. Analysis of these sequences, as well as those of the previously reported Chinese hamster and rat poly-u genes, supports the assertion that the homogenization of the ubiquitin-C gene in rodents is due to unequal crossing-over events. The sequence divergence of noncoding DNA was used to estimate the frequency of unequal crossing-over events (6.3 x 10-5 events per generation) in the Ubc gene, as well as to provide evidence of apparent selection in the poly-u gene.
Journal of Molecular Evolution
We are thankful to J.A. Southerland and P.L. Jiang for technical assistance in DNA sequencing, as well as to Y.I. Pavlov for helpful discussions. This work was supported by public Health Service Research Grant AI45135 from the Institute of Allergy and Infectious Diseases, National Institutes of Health.
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Perelygin A, Kondrashov F, Rogozin I, Brinton M. Evolution of the mouse polyubiquitin C gene. Journal of Molecular Evolution. 2002;55(2):202-210. doi:10.1007/s00239-002-2318-0
Perelygin, A., Kondrashov, F., Rogozin, I., & Brinton, M. (2002). Evolution of the mouse polyubiquitin C gene. Journal of Molecular Evolution, 55(2), 202–210. https://doi.org/10.1007/s00239-002-2318-0
Perelygin, Andrey, Fyodor Kondrashov, Igor Rogozin, and Margo Brinton. “Evolution of the Mouse Polyubiquitin C Gene.” Journal of Molecular Evolution 55, no. 2 (2002): 202–10. https://doi.org/10.1007/s00239-002-2318-0.
A. Perelygin, F. Kondrashov, I. Rogozin, and M. Brinton, “Evolution of the mouse polyubiquitin C gene,” Journal of Molecular Evolution, vol. 55, no. 2, pp. 202–210, 2002.
Perelygin A, Kondrashov F, Rogozin I, Brinton M. 2002. Evolution of the mouse polyubiquitin C gene. Journal of Molecular Evolution. 55(2), 202–210.
Perelygin, Andrey, et al. “Evolution of the Mouse Polyubiquitin C Gene.” Journal of Molecular Evolution, vol. 55, no. 2, Springer, 2002, pp. 202–10, doi:10.1007/s00239-002-2318-0.