Novel insights into PIN polarity regulation during Arabidopsis development

H. Han, Novel Insights into PIN Polarity Regulation during Arabidopsis Development, IST Austria, 2020.

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Thesis | Published | English
Department
Series Title
IST Austria Thesis
Abstract
The plant hormone auxin plays indispensable roles in plant growth and development. An essential level of regulation in auxin action is the directional auxin transport within cells. The establishment of auxin gradient in plant tissue has been attributed to local auxin biosynthesis and directional intercellular auxin transport, which both are controlled by various environmental and developmental signals. It is well established that asymmetric auxin distribution in cells is achieved by polarly localized PIN-FORMED (PIN) auxin efflux transporters. Despite the initial insights into cellular mechanisms of PIN polarization obtained from the last decades, the molecular mechanism and specific regulators mediating PIN polarization remains elusive. In this thesis, we aim to find novel players in PIN subcellular polarity regulation during Arabidopsis development. We first characterize the physiological effect of piperonylic acid (PA) on Arabidopsis hypocotyl gravitropic bending and PIN polarization. Secondly, we reveal the importance of SCFTIR1/AFB auxin signaling pathway in shoot gravitropism bending termination. In addition, we also explore the role of myosin XI complex, and actin cytoskeleton in auxin feedback regulation on PIN polarity. In Chapter 1, we give an overview of the current knowledge about PIN-mediated auxin fluxes in various plant tropic responses. In Chapter 2, we study the physiological effect of PA on shoot gravitropic bending. Our results show that PA treatment inhibits auxin-mediated PIN3 repolarization by interfering with PINOID and PIN3 phosphorylation status, ultimately leading to hyperbending hypocotyls. In Chapter 3, we provide evidence to show that the SCFTIR1/AFB nuclear auxin signaling pathway is crucial and required for auxin-mediated PIN3 repolarization and shoot gravitropic bending termination. In Chapter 4, we perform a phosphoproteomics approach and identify the motor protein Myosin XI and its binding protein, the MadB2 family, as an essential regulator of PIN polarity for auxin-canalization related developmetal processes. In Chapter 5, we demonstrate the vital role of actin cytoskeleton in auxin feedback on PIN polarity by regulating PIN subcellular trafficking. Overall, the data presented in this PhD thesis brings novel insights into the PIN polar localization regulation that resulted in the (re)establishment of the polar auxin flow and gradient in response to environmental stimuli during plant development.
Publishing Year
Date Published
2020-09-30
Acknowledgement
I also want to thank the China Scholarship Council for supporting my study during the year from 2015 to 2019. I also want to thank IST facilities – the Bioimaging facility, the media kitchen, the plant facility and all of the campus services, for their support.
Page
164
IST-REx-ID

Cite this

Han H. Novel Insights into PIN Polarity Regulation during Arabidopsis Development. IST Austria; 2020. doi:10.15479/AT:ISTA:8589
Han, H. (2020). Novel insights into PIN polarity regulation during Arabidopsis development. IST Austria. https://doi.org/10.15479/AT:ISTA:8589
Han, Huibin. Novel Insights into PIN Polarity Regulation during Arabidopsis Development. IST Austria, 2020. https://doi.org/10.15479/AT:ISTA:8589.
H. Han, Novel insights into PIN polarity regulation during Arabidopsis development. IST Austria, 2020.
Han H. 2020. Novel insights into PIN polarity regulation during Arabidopsis development, IST Austria, 164p.
Han, Huibin. Novel Insights into PIN Polarity Regulation during Arabidopsis Development. IST Austria, 2020, doi:10.15479/AT:ISTA:8589.
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2020-09-30
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