Hadamard frequency-encoded SOFAST-HMQC for ultrafast two-dimensional protein NMR

P. Schanda, B. Brutscher, Journal of Magnetic Resonance 178 (2006) 334–339.

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Journal Article | Published | English
Author
Schanda, PaulIST Austria ; Brutscher, Bernhard
Abstract
We demonstrate the feasibility of recording 1H–15N correlation spectra of proteins in only one second of acquisition time. The experiment combines recently proposed SOFAST-HMQC with Hadamard-type 15N frequency encoding. This allows site-resolved real-time NMR studies of kinetic processes in proteins with an increased time resolution. The sensitivity of the experiment is sufficient to be applicable to a wide range of molecular systems available at millimolar concentration on a high magnetic field spectrometer.
Publishing Year
Date Published
2006-02-01
Journal Title
Journal of Magnetic Resonance
Volume
178
Issue
2
Page
334-339
ISSN
IST-REx-ID

Cite this

Schanda P, Brutscher B. Hadamard frequency-encoded SOFAST-HMQC for ultrafast two-dimensional protein NMR. Journal of Magnetic Resonance. 2006;178(2):334-339. doi:10.1016/j.jmr.2005.10.007
Schanda, P., & Brutscher, B. (2006). Hadamard frequency-encoded SOFAST-HMQC for ultrafast two-dimensional protein NMR. Journal of Magnetic Resonance, 178(2), 334–339. https://doi.org/10.1016/j.jmr.2005.10.007
Schanda, Paul, and Bernhard Brutscher. “Hadamard Frequency-Encoded SOFAST-HMQC for Ultrafast Two-Dimensional Protein NMR.” Journal of Magnetic Resonance 178, no. 2 (2006): 334–39. https://doi.org/10.1016/j.jmr.2005.10.007.
P. Schanda and B. Brutscher, “Hadamard frequency-encoded SOFAST-HMQC for ultrafast two-dimensional protein NMR,” Journal of Magnetic Resonance, vol. 178, no. 2, pp. 334–339, 2006.
Schanda P, Brutscher B. 2006. Hadamard frequency-encoded SOFAST-HMQC for ultrafast two-dimensional protein NMR. Journal of Magnetic Resonance. 178(2), 334–339.
Schanda, Paul, and Bernhard Brutscher. “Hadamard Frequency-Encoded SOFAST-HMQC for Ultrafast Two-Dimensional Protein NMR.” Journal of Magnetic Resonance, vol. 178, no. 2, Elsevier, 2006, pp. 334–39, doi:10.1016/j.jmr.2005.10.007.

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