--- res: bibo_abstract: - High signal to noise is a necessity for the quantification of NMR spectral parameters to be translated into accurate and precise restraints on protein structure and dynamics. An important source of long-range structural information is obtained from 1H–1H residual dipolar couplings (RDCs) measured for weakly aligned molecules. For sensitivity reasons, such measurements are generally performed on highly deuterated protein samples. Here we show that high sensitivity is also obtained for protonated protein samples if the pulse schemes are optimized in terms of longitudinal relaxation efficiency and J-mismatch compensated coherence transfer. The new sensitivity-optimized quantitative J-correlation experiment yields important signal gains reaching factors of 1.5 to 8 for individual correlation peaks when compared to previously proposed pulse schemes.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Paul foaf_name: Schanda, Paul foaf_surname: Schanda foaf_workInfoHomepage: http://www.librecat.org/personId=7B541462-FAF6-11E9-A490-E8DFE5697425 orcid: 0000-0002-9350-7606 - foaf_Person: foaf_givenName: Ewen foaf_name: Lescop, Ewen foaf_surname: Lescop - foaf_Person: foaf_givenName: Mirjam foaf_name: Falge, Mirjam foaf_surname: Falge - foaf_Person: foaf_givenName: Rémy foaf_name: Sounier, Rémy foaf_surname: Sounier - foaf_Person: foaf_givenName: Jérôme foaf_name: Boisbouvier, Jérôme foaf_surname: Boisbouvier - foaf_Person: foaf_givenName: Bernhard foaf_name: Brutscher, Bernhard foaf_surname: Brutscher bibo_doi: 10.1007/s10858-006-9138-2 bibo_volume: 38 dct_date: 2007^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/0925-2738 - http://id.crossref.org/issn/1573-5001 dct_language: eng dct_publisher: Springer Nature@ dct_subject: - Spectroscopy - Biochemistry dct_title: Sensitivity-optimized experiment for the measurement of residual dipolar couplings between amide protons@ ...