A set of BEST triple-resonance experiments for time-optimized protein resonance assignment

Lescop E, Schanda P, Brutscher B. 2007. A set of BEST triple-resonance experiments for time-optimized protein resonance assignment. Journal of Magnetic Resonance. 187(1), 163–169.

Download
No fulltext has been uploaded. References only!

Journal Article | Published | English
Author
Lescop, Ewen; Schanda, PaulIST Austria ; Brutscher, Bernhard
Abstract
A series of sequential, intra-residue, and bi-directional BEST H–N–CA, H–N–CO, and H–N–CB pulse sequences is presented that extends the BEST concept introduced recently for fast multidimensional protein NMR [Schanda et al., J. Am. Chem. Soc. 128 (2006) 9042] to the complete set of experiments required for sequential resonance assignment. We demonstrate for the protein ubiquitin that 3D BEST H–N–C correlation spectra can be recorded on a 600 MHz NMR spectrometer equipped with a cryogenic probe in only a few minutes of acquisition time with sufficient sensitivity to detect all expected cross peaks.
Publishing Year
Date Published
2007-07-01
Journal Title
Journal of Magnetic Resonance
Volume
187
Issue
1
Page
163-169
ISSN
IST-REx-ID

Cite this

Lescop E, Schanda P, Brutscher B. A set of BEST triple-resonance experiments for time-optimized protein resonance assignment. Journal of Magnetic Resonance. 2007;187(1):163-169. doi:10.1016/j.jmr.2007.04.002
Lescop, E., Schanda, P., & Brutscher, B. (2007). A set of BEST triple-resonance experiments for time-optimized protein resonance assignment. Journal of Magnetic Resonance. Elsevier. https://doi.org/10.1016/j.jmr.2007.04.002
Lescop, Ewen, Paul Schanda, and Bernhard Brutscher. “A Set of BEST Triple-Resonance Experiments for Time-Optimized Protein Resonance Assignment.” Journal of Magnetic Resonance. Elsevier, 2007. https://doi.org/10.1016/j.jmr.2007.04.002.
E. Lescop, P. Schanda, and B. Brutscher, “A set of BEST triple-resonance experiments for time-optimized protein resonance assignment,” Journal of Magnetic Resonance, vol. 187, no. 1. Elsevier, pp. 163–169, 2007.
Lescop E, Schanda P, Brutscher B. 2007. A set of BEST triple-resonance experiments for time-optimized protein resonance assignment. Journal of Magnetic Resonance. 187(1), 163–169.
Lescop, Ewen, et al. “A Set of BEST Triple-Resonance Experiments for Time-Optimized Protein Resonance Assignment.” Journal of Magnetic Resonance, vol. 187, no. 1, Elsevier, 2007, pp. 163–69, doi:10.1016/j.jmr.2007.04.002.

Export

Marked Publications

Open Data IST Research Explorer

Search this title in

Google Scholar