Methyl-specific isotope labeling strategies for NMR studies of membrane proteins

V. Kurauskas, P. Schanda, R. Sounier, in:, Membrane Protein Structure and Function Characterization, Springer Nature, 2017, pp. 109–123.

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Book Chapter | Published | English
Author
Kurauskas, Vilius; Schanda, PaulIST Austria ; Sounier, Remy
Series Title
Methods in Molecular Biology
Abstract
Methyl groups are very useful probes of structure, dynamics, and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter, we discuss the approach for labeling methyl groups in E. coli-based protein expression, as exemplified with the mitochondrial carrier GGC.
Publishing Year
Date Published
2017-07-29
Book Title
Membrane protein structure and function characterization
Volume
1635
Page
109-123
IST-REx-ID

Cite this

Kurauskas V, Schanda P, Sounier R. Methyl-specific isotope labeling strategies for NMR studies of membrane proteins. In: Membrane Protein Structure and Function Characterization. Vol 1635. Springer Nature; 2017:109-123. doi:10.1007/978-1-4939-7151-0_6
Kurauskas, V., Schanda, P., & Sounier, R. (2017). Methyl-specific isotope labeling strategies for NMR studies of membrane proteins. In Membrane protein structure and function characterization (Vol. 1635, pp. 109–123). Springer Nature. https://doi.org/10.1007/978-1-4939-7151-0_6
Kurauskas, Vilius, Paul Schanda, and Remy Sounier. “Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.” In Membrane Protein Structure and Function Characterization, 1635:109–23. Springer Nature, 2017. https://doi.org/10.1007/978-1-4939-7151-0_6.
V. Kurauskas, P. Schanda, and R. Sounier, “Methyl-specific isotope labeling strategies for NMR studies of membrane proteins,” in Membrane protein structure and function characterization, vol. 1635, Springer Nature, 2017, pp. 109–123.
Kurauskas V, Schanda P, Sounier R. 2017. Methyl-specific isotope labeling strategies for NMR studies of membrane proteins. Membrane protein structure and function characterization. , Methods in Molecular Biology, vol. 1635. 109–123.
Kurauskas, Vilius, et al. “Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.” Membrane Protein Structure and Function Characterization, vol. 1635, Springer Nature, 2017, pp. 109–23, doi:10.1007/978-1-4939-7151-0_6.

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