--- res: bibo_abstract: - 'Studying protein dynamics on microsecond‐to‐millisecond (μs‐ms) time scales can provide important insight into protein function. In magic‐angle‐spinning (MAS) NMR, μs dynamics can be visualized by R1p rotating‐frame relaxation dispersion experiments in different regimes of radio‐frequency field strengths: at low RF field strength, isotropic‐chemical‐shift fluctuation leads to “Bloch‐McConnell‐type” relaxation dispersion, while when the RF field approaches rotary resonance conditions bond angle fluctuations manifest as increased R1p rate constants (“Near‐Rotary‐Resonance Relaxation Dispersion”, NERRD). Here we explore the joint analysis of both regimes to gain comprehensive insight into motion in terms of geometric amplitudes, chemical‐shift changes, populations and exchange kinetics. We use a numerical simulation procedure to illustrate these effects and the potential of extracting exchange parameters, and apply the methodology to the study of a previously described conformational exchange process in microcrystalline ubiquitin.@eng' bibo_authorlist: - foaf_Person: foaf_givenName: Dominique foaf_name: Marion, Dominique foaf_surname: Marion - foaf_Person: foaf_givenName: Diego F. foaf_name: Gauto, Diego F. foaf_surname: Gauto - foaf_Person: foaf_givenName: Isabel foaf_name: Ayala, Isabel foaf_surname: Ayala - foaf_Person: foaf_givenName: Karine foaf_name: Giandoreggio-Barranco, Karine foaf_surname: Giandoreggio-Barranco - foaf_Person: foaf_givenName: Paul foaf_name: Schanda, Paul foaf_surname: Schanda foaf_workInfoHomepage: http://www.librecat.org/personId=7B541462-FAF6-11E9-A490-E8DFE5697425 orcid: 0000-0002-9350-7606 bibo_doi: 10.1002/cphc.201800935 bibo_issue: '2' bibo_volume: 20 dct_date: 2019^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/1439-4235 dct_language: eng dct_publisher: Wiley@ dct_subject: - Physical and Theoretical Chemistry - Atomic and Molecular Physics - and Optics dct_title: Microsecond protein dynamics from combined Bloch-McConnell and Near-Rotary-Resonance R1p relaxation-dispersion MAS NMR@ fabio_hasPubmedId: '30444575' ...