--- res: bibo_abstract: - "Background: The mitochondrial pyruvate carrier (MPC) plays a central role in energy metabolism by transporting pyruvate across the inner mitochondrial membrane. Its heterodimeric composition and homology to SWEET and semiSWEET transporters set the MPC apart from the canonical mitochondrial carrier family (named MCF or SLC25). The import of the canonical carriers is mediated by the carrier translocase of the inner membrane (TIM22) pathway and is dependent on their structure, which features an even number of transmembrane segments and both termini in the intermembrane space. The import pathway of MPC proteins has not been elucidated. The odd number of transmembrane segments and positioning of the N-terminus in the matrix argues against an import via the TIM22 carrier pathway but favors an import via the flexible presequence pathway.\r\nResults: Here, we systematically analyzed the import pathways of Mpc2 and Mpc3 and report that, contrary to an expected import via the flexible presequence pathway, yeast MPC proteins with an odd number of transmembrane segments and matrix-exposed N-terminus are imported by the carrier pathway, using the receptor Tom70, small TIM chaperones, and the TIM22 complex. The TIM9ยท10 complex chaperones MPC proteins through the mitochondrial intermembrane space using conserved hydrophobic motifs that are also required for the interaction with canonical carrier proteins.\r\nConclusions: The carrier pathway can import paired and non-paired transmembrane helices and translocate N-termini to either side of the mitochondrial inner membrane, revealing an unexpected versatility of the mitochondrial import pathway for non-cleavable inner membrane proteins.@eng" bibo_authorlist: - foaf_Person: foaf_givenName: Heike foaf_name: Rampelt, Heike foaf_surname: Rampelt - foaf_Person: foaf_givenName: Iva foaf_name: Sucec, Iva foaf_surname: Sucec - foaf_Person: foaf_givenName: Beate foaf_name: Bersch, Beate foaf_surname: Bersch - foaf_Person: foaf_givenName: Patrick foaf_name: Horten, Patrick foaf_surname: Horten - foaf_Person: foaf_givenName: Inge foaf_name: Perschil, Inge foaf_surname: Perschil - foaf_Person: foaf_givenName: Jean-Claude foaf_name: Martinou, Jean-Claude foaf_surname: Martinou - foaf_Person: foaf_givenName: Martin foaf_name: van der Laan, Martin foaf_surname: van der Laan - foaf_Person: foaf_givenName: Nils foaf_name: Wiedemann, Nils foaf_surname: Wiedemann - foaf_Person: foaf_givenName: Paul foaf_name: Schanda, Paul foaf_surname: Schanda foaf_workInfoHomepage: http://www.librecat.org/personId=7B541462-FAF6-11E9-A490-E8DFE5697425 orcid: 0000-0002-9350-7606 - foaf_Person: foaf_givenName: Nikolaus foaf_name: Pfanner, Nikolaus foaf_surname: Pfanner bibo_doi: 10.1186/s12915-019-0733-6 bibo_volume: 18 dct_date: 2020^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/1741-7007 dct_language: eng dct_publisher: Springer Nature@ dct_subject: - Biotechnology - Plant Science - General Biochemistry - Genetics and Molecular Biology - Developmental Biology - Cell Biology - Physiology - Ecology - Evolution - Behavior and Systematics - Structural Biology - General Agricultural and Biological Sciences dct_title: The mitochondrial carrier pathway transports non-canonical substrates with an odd number of transmembrane segments@ fabio_hasPubmedId: '31907035' ...