---
_id: '813'
abstract:
- lang: eng
text: The Gag polyprotein of retroviruses drives immature virus assembly by forming
hexameric protein lattices. The assembly is primarily mediated by protein-protein
interactions between capsid (CA) domains and by interactions between nucleocapsid
(NC) domains and RNA. Specific interactions between NC and the viral RNA are required
for genome packaging. Previously reported cryoelectron microscopy analysis of
immature Mason-Pfizer monkey virus (M-PMV) particles suggested that a basic region
(residues RKK) in CA may serve as an additional binding site for nucleic acids.
Here, we have introduced mutations into the RKK region in both bacterial and proviral
M-PMV vectors and have assessed their impact on M-PMV assembly, structure, RNA
binding, budding/release, nuclear trafficking, and infectivity using in vitro
and in vivo systems. Our data indicate that the RKK region binds and structures
nucleic acid that serves to promote virus particle assembly in the cytoplasm.
Moreover, the RKK region appears to be important for recruitment of viral genomic
RNA into Gag particles, and this function could be linked to changes in nuclear
trafficking. Together these observations suggest that in M-PMV, direct interactions
between CA and nucleic acid play important functions in the late stages of the
viral life cycle.
acknowledgement: |-
Work in the laboratory of John A. G. Briggs was funded by Deutsche
Forschungsgemeinschaft (DFG) (BR 3635/2-1). This work, including the
efforts of Tomas Ruml, was funded by the Grant Agency of the Czech
Republic (14-15326S) and the Czech Ministry of Education (NPU I sus-
tainability projects LO1302 and LO1304).
author:
- first_name: Tibor
full_name: Füzik, Tibor
last_name: Füzik
- first_name: Růžena
full_name: ' Píchalová, Růžena'
last_name: Píchalová
- first_name: Florian
full_name: Florian Schur
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
- first_name: Karolína
full_name: Strohalmová, Karolína
last_name: Strohalmová
- first_name: Ivana
full_name: Křížová, Ivana
last_name: Křížová
- first_name: Romana
full_name: Hadravová, Romana
last_name: Hadravová
- first_name: Michaela
full_name: Rumlová, Michaela
last_name: Rumlová
- first_name: John
full_name: Briggs, John A
last_name: Briggs
- first_name: Pavel
full_name: Ulbrich, Pavel
last_name: Ulbrich
- first_name: Tomáš
full_name: Ruml, Tomáš
last_name: Ruml
citation:
ama: Füzik T, Píchalová R, Schur FK, et al. Nucleic acid binding by Mason-Pfizer
monkey virus CA promotes virus assembly and genome packaging. Journal of Virology.
2016;90(9):4593-4603. doi:10.1128/JVI.03197-15
apa: Füzik, T., Píchalová, R., Schur, F. K., Strohalmová, K., Křížová, I., Hadravová,
R., … Ruml, T. (2016). Nucleic acid binding by Mason-Pfizer monkey virus CA promotes
virus assembly and genome packaging. Journal of Virology. ASM. https://doi.org/10.1128/JVI.03197-15
chicago: Füzik, Tibor, Růžena Píchalová, Florian KM Schur, Karolína Strohalmová,
Ivana Křížová, Romana Hadravová, Michaela Rumlová, John Briggs, Pavel Ulbrich,
and Tomáš Ruml. “Nucleic Acid Binding by Mason-Pfizer Monkey Virus CA Promotes
Virus Assembly and Genome Packaging.” Journal of Virology. ASM, 2016. https://doi.org/10.1128/JVI.03197-15.
ieee: T. Füzik et al., “Nucleic acid binding by Mason-Pfizer monkey virus
CA promotes virus assembly and genome packaging,” Journal of Virology,
vol. 90, no. 9. ASM, pp. 4593–4603, 2016.
ista: Füzik T, Píchalová R, Schur FK, Strohalmová K, Křížová I, Hadravová R, Rumlová
M, Briggs J, Ulbrich P, Ruml T. 2016. Nucleic acid binding by Mason-Pfizer monkey
virus CA promotes virus assembly and genome packaging. Journal of Virology. 90(9),
4593–4603.
mla: Füzik, Tibor, et al. “Nucleic Acid Binding by Mason-Pfizer Monkey Virus CA
Promotes Virus Assembly and Genome Packaging.” Journal of Virology, vol.
90, no. 9, ASM, 2016, pp. 4593–603, doi:10.1128/JVI.03197-15.
short: T. Füzik, R. Píchalová, F.K. Schur, K. Strohalmová, I. Křížová, R. Hadravová,
M. Rumlová, J. Briggs, P. Ulbrich, T. Ruml, Journal of Virology 90 (2016) 4593–4603.
date_created: 2018-12-11T11:48:38Z
date_published: 2016-05-01T00:00:00Z
date_updated: 2021-01-12T08:17:03Z
day: '01'
doi: 10.1128/JVI.03197-15
extern: 1
intvolume: ' 90'
issue: '9'
month: '05'
page: 4593 - 4603
publication: Journal of Virology
publication_status: published
publisher: ASM
publist_id: '6835'
quality_controlled: 0
status: public
title: Nucleic acid binding by Mason-Pfizer monkey virus CA promotes virus assembly
and genome packaging
type: journal_article
volume: 90
year: '2016'
...