Single-molecule electrophoresis of β-hairpin peptides by electrical recordings and Langevin dynamics simulations

Goodrich CP, Kirmizialtin S, Huyghues-Despointes BM, Zhu A, Scholtz JM, Makarov DE, Movileanu L. 2007. Single-molecule electrophoresis of β-hairpin peptides by electrical recordings and Langevin dynamics simulations. The Journal of Physical Chemistry B. 111(13), 3332–3335.

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Journal Article | Published | English
Author
Goodrich, Carl PeterIST Austria ; Kirmizialtin, Serdal; Huyghues-Despointes, Beatrice M.; Zhu, Aiping; Scholtz, J. Martin; Makarov, Dmitrii E.; Movileanu, Liviu
Abstract
We used single-channel electrical recordings and Langevin molecular dynamics simulations to explore the electrophoretic translocation of various β-hairpin peptides across the staphylococcal α-hemolysin (αHL) protein pore at single-molecule resolution. The β-hairpin peptides, which varied in their folding properties, corresponded to the C terminal residues of the B1 domain of protein G. The translocation time was strongly dependent on the electric force and was correlated with the folding features of the β-hairpin peptides. Highly unfolded peptides entered the pore in an extended conformation, resulting in fast single-file translocation events. In contrast, the translocation of the folded β-hairpin peptides occurred more slowly. In this case, the β-hairpin peptides traversed the αHL pore in a misfolded or fully folded conformation. This study demonstrates that the interaction between a polypeptide and a β-barrel protein pore is dependent on the folding features of the polypeptide.
Publishing Year
Date Published
2007-03-13
Journal Title
The Journal of Physical Chemistry B
Volume
111
Issue
13
Page
3332-3335
IST-REx-ID

Cite this

Goodrich CP, Kirmizialtin S, Huyghues-Despointes BM, et al. Single-molecule electrophoresis of β-hairpin peptides by electrical recordings and Langevin dynamics simulations. The Journal of Physical Chemistry B. 2007;111(13):3332-3335. doi:10.1021/jp071364h
Goodrich, C. P., Kirmizialtin, S., Huyghues-Despointes, B. M., Zhu, A., Scholtz, J. M., Makarov, D. E., & Movileanu, L. (2007). Single-molecule electrophoresis of β-hairpin peptides by electrical recordings and Langevin dynamics simulations. The Journal of Physical Chemistry B. American Chemical Society. https://doi.org/10.1021/jp071364h
Goodrich, Carl Peter, Serdal Kirmizialtin, Beatrice M. Huyghues-Despointes, Aiping Zhu, J. Martin Scholtz, Dmitrii E. Makarov, and Liviu Movileanu. “Single-Molecule Electrophoresis of β-Hairpin Peptides by Electrical Recordings and Langevin Dynamics Simulations.” The Journal of Physical Chemistry B. American Chemical Society, 2007. https://doi.org/10.1021/jp071364h.
C. P. Goodrich et al., “Single-molecule electrophoresis of β-hairpin peptides by electrical recordings and Langevin dynamics simulations,” The Journal of Physical Chemistry B, vol. 111, no. 13. American Chemical Society, pp. 3332–3335, 2007.
Goodrich CP, Kirmizialtin S, Huyghues-Despointes BM, Zhu A, Scholtz JM, Makarov DE, Movileanu L. 2007. Single-molecule electrophoresis of β-hairpin peptides by electrical recordings and Langevin dynamics simulations. The Journal of Physical Chemistry B. 111(13), 3332–3335.
Goodrich, Carl Peter, et al. “Single-Molecule Electrophoresis of β-Hairpin Peptides by Electrical Recordings and Langevin Dynamics Simulations.” The Journal of Physical Chemistry B, vol. 111, no. 13, American Chemical Society, 2007, pp. 3332–35, doi:10.1021/jp071364h.

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