--- res: bibo_abstract: - Glutamate is the major excitatory neurotransmitter in the CNS binding to a variety of glutamate receptors. Metabotropic glutamate receptors (mGluR1 to mGluR8) can act excitatory or inhibitory, depending on associated signal cascades. Expression and localization of inhibitory acting mGluRs at inner hair cells (IHCs) in the cochlea are largely unknown. Here, we analyzed expression of mGluR2, mGluR3, mGluR4, mGluR6, mGluR7, and mGluR8 and investigated their localization with respect to the presynaptic ribbon of IHC synapses. We detected transcripts for mGluR2, mGluR3, and mGluR4 as well as for mGluR7a, mGluR7b, mGluR8a, and mGluR8b splice variants. Using receptor-specific antibodies in cochlear wholemounts, we found expression of mGluR2, mGluR4, and mGluR8b close to presynaptic ribbons. Super resolution and confocal microscopy in combination with 3-dimensional reconstructions indicated a postsynaptic localization of mGluR2 that overlaps with postsynaptic density protein 95 on dendrites of afferent type I spiral ganglion neurons. In contrast, mGluR4 and mGluR8b were expressed at the presynapse close to IHC ribbons. In summary, we localized in detail 3 mGluR types at IHC ribbon synapses, providing a fundament for new therapeutical strategies that could protect the cochlea against noxious stimuli and excitotoxicity.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Lisa foaf_name: Klotz, Lisa foaf_surname: Klotz - foaf_Person: foaf_givenName: Olaf foaf_name: Wendler, Olaf foaf_surname: Wendler - foaf_Person: foaf_givenName: Renato foaf_name: Frischknecht, Renato foaf_surname: Frischknecht - foaf_Person: foaf_givenName: Ryuichi foaf_name: Shigemoto, Ryuichi foaf_surname: Shigemoto foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8761-9444 - foaf_Person: foaf_givenName: Holger foaf_name: Schulze, Holger foaf_surname: Schulze - foaf_Person: foaf_givenName: Ralf foaf_name: Enz, Ralf foaf_surname: Enz bibo_doi: 10.1096/fj.201901543R bibo_issue: '12' bibo_volume: 33 dct_date: 2019^xs_gYear dct_identifier: - UT:000507466100054 dct_isPartOf: - http://id.crossref.org/issn/15306860 dct_language: eng dct_publisher: FASEB@ dct_title: Localization of group II and III metabotropic glutamate receptors at pre- and postsynaptic sites of inner hair cell ribbon synapses@ fabio_hasPubmedId: '31585509' ...