{"day":"01","publisher":"Elsevier","department":[{"_id":"GaNo"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","pmid":1,"publication_status":"published","language":[{"iso":"eng"}],"publication":"Drug and Alcohol Dependence","_id":"714","oa":1,"scopus_import":1,"abstract":[{"lang":"eng","text":"Background HIV-1 infection and drug abuse are frequently co-morbid and their association greatly increases the severity of HIV-1-induced neuropathology. While nucleus accumbens (NAcc) function is severely perturbed by drugs of abuse, little is known about how HIV-1 infection affects NAcc. Methods We used calcium and voltage imaging to investigate the effect of HIV-1 trans-activator of transcription (Tat) on rat NAcc. Based on previous neuronal studies, we hypothesized that Tat modulates intracellular Ca2+ homeostasis of NAcc neurons. Results We provide evidence that Tat triggers a Ca2+ signaling cascade in NAcc medium spiny neurons (MSN) expressing D1-like dopamine receptors leading to neuronal depolarization. Firstly, Tat induced inositol 1,4,5-trisphsophate (IP3) receptor-mediated Ca2+ release from endoplasmic reticulum, followed by Ca2+ and Na+ influx via transient receptor potential canonical channels. The influx of cations depolarizes the membrane promoting additional Ca2+ entry through voltage-gated P/Q-type Ca2+ channels and opening of tetrodotoxin-sensitive Na+ channels. By activating this mechanism, Tat elicits a feed-forward depolarization increasing the excitability of D1-phosphatidylinositol-linked NAcc MSN. We previously found that cocaine targets NAcc neurons directly (independent of the inhibition of dopamine transporter) only when IP3-generating mechanisms are concomitantly initiated. When tested here, cocaine produced a dose-dependent potentiation of the effect of Tat on cytosolic Ca2+. Conclusion We describe for the first time a HIV-1 Tat-triggered Ca2+ signaling in MSN of NAcc involving TRPC and depolarization and a potentiation of the effect of Tat by cocaine, which may be relevant for the reward axis in cocaine-abusing HIV-1-positive patients."}],"volume":178,"date_created":"2018-12-11T11:48:05Z","publication_identifier":{"issn":["03768716"]},"month":"09","year":"2017","intvolume":"       178","citation":{"ieee":"G. Brailoiu <i>et al.</i>, “HIV Tat excites D1 receptor-like expressing neurons from rat nucleus accumbens,” <i>Drug and Alcohol Dependence</i>, vol. 178. Elsevier, pp. 7–14, 2017.","ama":"Brailoiu G, Deliu E, Barr J, et al. HIV Tat excites D1 receptor-like expressing neurons from rat nucleus accumbens. <i>Drug and Alcohol Dependence</i>. 2017;178:7-14. doi:<a href=\"https://doi.org/10.1016/j.drugalcdep.2017.04.015\">10.1016/j.drugalcdep.2017.04.015</a>","short":"G. Brailoiu, E. Deliu, J. Barr, L. Console Bram, A. Ciuciu, M. Abood, E. Unterwald, E. Brǎiloiu, Drug and Alcohol Dependence 178 (2017) 7–14.","mla":"Brailoiu, Gabriela, et al. “HIV Tat Excites D1 Receptor-like Expressing Neurons from Rat Nucleus Accumbens.” <i>Drug and Alcohol Dependence</i>, vol. 178, Elsevier, 2017, pp. 7–14, doi:<a href=\"https://doi.org/10.1016/j.drugalcdep.2017.04.015\">10.1016/j.drugalcdep.2017.04.015</a>.","apa":"Brailoiu, G., Deliu, E., Barr, J., Console Bram, L., Ciuciu, A., Abood, M., … Brǎiloiu, E. (2017). HIV Tat excites D1 receptor-like expressing neurons from rat nucleus accumbens. <i>Drug and Alcohol Dependence</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.drugalcdep.2017.04.015\">https://doi.org/10.1016/j.drugalcdep.2017.04.015</a>","ista":"Brailoiu G, Deliu E, Barr J, Console Bram L, Ciuciu A, Abood M, Unterwald E, Brǎiloiu E. 2017. HIV Tat excites D1 receptor-like expressing neurons from rat nucleus accumbens. Drug and Alcohol Dependence. 178, 7–14.","chicago":"Brailoiu, Gabriela, Elena Deliu, Jeffrey Barr, Linda Console Bram, Alexandra Ciuciu, Mary Abood, Ellen Unterwald, and Eugen Brǎiloiu. “HIV Tat Excites D1 Receptor-like Expressing Neurons from Rat Nucleus Accumbens.” <i>Drug and Alcohol Dependence</i>. Elsevier, 2017. <a href=\"https://doi.org/10.1016/j.drugalcdep.2017.04.015\">https://doi.org/10.1016/j.drugalcdep.2017.04.015</a>."},"date_published":"2017-09-01T00:00:00Z","quality_controlled":"1","title":"HIV Tat excites D1 receptor-like expressing neurons from rat nucleus accumbens","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797705"}],"acknowledgement":"This work was supported by the National Institutes of Health grants DA035926 (to MEA), and P30DA013429 (to EMU).","page":"7 - 14","doi":"10.1016/j.drugalcdep.2017.04.015","type":"journal_article","article_type":"original","oa_version":"Submitted Version","publist_id":"6967","date_updated":"2021-01-12T08:12:00Z","status":"public","external_id":{"pmid":["28623807"]},"author":[{"full_name":"Brailoiu, Gabriela","last_name":"Brailoiu","first_name":"Gabriela"},{"full_name":"Deliu, Elena","id":"37A40D7E-F248-11E8-B48F-1D18A9856A87","first_name":"Elena","orcid":"0000-0002-7370-5293","last_name":"Deliu"},{"last_name":"Barr","first_name":"Jeffrey","full_name":"Barr, Jeffrey"},{"full_name":"Console Bram, Linda","last_name":"Console Bram","first_name":"Linda"},{"full_name":"Ciuciu, Alexandra","last_name":"Ciuciu","first_name":"Alexandra"},{"full_name":"Abood, Mary","first_name":"Mary","last_name":"Abood"},{"full_name":"Unterwald, Ellen","first_name":"Ellen","last_name":"Unterwald"},{"last_name":"Brǎiloiu","first_name":"Eugen","full_name":"Brǎiloiu, Eugen"}]}