Data for the paper "Gene amplification as a form of population-level gene expression regulation"

I. Tomanek, Data for the Paper “Gene Amplification as a Form of Population-Level Gene Expression Regulation,” IST Austria, 2019.

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Research Data
Department
Abstract
Organisms cope with change by employing transcriptional regulators. However, when faced with rare environments, the evolution of transcriptional regulators and their promoters may be too slow. We ask whether the intrinsic instability of gene duplication and amplification provides a generic alternative to canonical gene regulation. By real-time monitoring of gene copy number mutations in E. coli, we show that gene duplications and amplifications enable adaptation to fluctuating environments by rapidly generating copy number, and hence expression level, polymorphism. This ‘amplification-mediated gene expression tuning’ occurs on timescales similar to canonical gene regulation and can deal with rapid environmental changes. Mathematical modeling shows that amplifications also tune gene expression in stochastic environments where transcription factor-based schemes are hard to evolve or maintain. The fleeting nature of gene amplifications gives rise to a generic population-level mechanism that relies on genetic heterogeneity to rapidly tune expression of any gene, without leaving any genomic signature.
Publishing Year
Date Published
2019-11-13
IST-REx-ID

Cite this

Tomanek I. Data for the Paper “Gene Amplification as a Form of Population-Level Gene Expression Regulation.” IST Austria; 2019. doi:10.15479/AT:ISTA:7016
Tomanek, I. (2019). Data for the paper “Gene amplification as a form of population-level gene expression regulation.” IST Austria. https://doi.org/10.15479/AT:ISTA:7016
Tomanek, Isabella. Data for the Paper “Gene Amplification as a Form of Population-Level Gene Expression Regulation.” IST Austria, 2019. https://doi.org/10.15479/AT:ISTA:7016.
I. Tomanek, Data for the paper “Gene amplification as a form of population-level gene expression regulation.” IST Austria, 2019.
Tomanek I. 2019. Data for the paper ‘Gene amplification as a form of population-level gene expression regulation’, IST Austria,p.
Tomanek, Isabella. Data for the Paper “Gene Amplification as a Form of Population-Level Gene Expression Regulation.” IST Austria, 2019, doi:10.15479/AT:ISTA:7016.
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Main File(s)
File Name
File Title
Locus1_amplified
Description
Illumina whole genome sequence data for Locus 1 - amplified.
Access Level
OA Open Access
Date Uploaded
2019-11-13
MD5 Checksum
72441055043eda4cbf1398a422e2c118
File Name
File Title
Locus1_ancestral
Description
Illumina whole genome sequence data for Locus 1 - ancestral.
Access Level
OA Open Access
Date Uploaded
2019-11-13
MD5 Checksum
a4ac50bf655d9c751f0305ade5c2ee16
File Name
File Title
Locus1_amplified_DOG
Description
Illumina whole genome sequence data for Locus 1 - amplified, after DOG-selection.
Access Level
OA Open Access
Date Uploaded
2019-11-13
MD5 Checksum
5b227708ff478ca06e3f0448a4efdc2f
File Name
File Title
Locus2_amplified
Description
Illumina whole genome sequence data for Locus 2 - amplified.
Access Level
OA Open Access
Date Uploaded
2019-11-13
MD5 Checksum
d9550a4c044116075fa83f8f2ea31d6f
File Name
File Title
Locus2_ancestral
Description
Illumina whole genome sequence data for Locus 2 - ancestral.
Access Level
OA Open Access
Date Uploaded
2019-11-13
MD5 Checksum
466ceb302c020ac013007a879fcde69d
File Name
File Title
Locus2_amplified_DOG
Description
Illumina whole genome sequence data for Locus 2 - amplified, after DOG-selection.
Access Level
OA Open Access
Date Uploaded
2019-11-21
MD5 Checksum
8aeb1da771713c7baa5a847eff889604
File Title
DNA sequence of the chromosomal reporter gene cassette
Description
Compressed genbank file format containing the sequence of the chromosomal reporter gene cassette.
Access Level
OA Open Access
Date Uploaded
2020-01-14
MD5 Checksum
bf7d4b053f14af4655fb5574209fdb2d
File Name
File Title
Read_me_sequence_data
Access Level
OA Open Access
Date Uploaded
2020-01-15
MD5 Checksum
5e91cee2eff6f4a7cde456c6fb07c2ff
File Name
File Title
FACS data
Description
FACS data associated with Fig. 2c - see read_me_FACS
Access Level
OA Open Access
Date Uploaded
2020-01-22
MD5 Checksum
5e6745dcfb9c1b11dd935ac3ee45fe33
File Name
Access Level
OA Open Access
Date Uploaded
2020-01-22
MD5 Checksum
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File Name
Access Level
OA Open Access
Date Uploaded
2020-01-22
MD5 Checksum
fd8ba5d75d24e47ddf7e70bfdadb40d4
File Name
File Title
microfluidics data
Description
microfluidics time trace data - see read_me_microfluidics
Access Level
OA Open Access
Date Uploaded
2020-01-22
MD5 Checksum
69c5dc5ca5c069a138183c934acc1778


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