Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase

L. Zhou, L.A. Sazanov, Science 365 (2019) eaaw9144.

Download
No fulltext has been uploaded. References only!

Journal Article | Published | English
Department
Abstract
V (vacuolar)/A (archaeal)-type adenosine triphosphatases (ATPases), found in archaeaand eubacteria, couple ATP hydrolysis or synthesis to proton translocation across theplasma membrane using the rotary-catalysis mechanism. They belong to the V-typeATPase family, which differs from the mitochondrial/chloroplast F-type ATP synthasesin overall architecture. We solved cryo–electron microscopy structures of the intactThermus thermophilusV/A-ATPase, reconstituted into lipid nanodiscs, in three rotationalstates and two substates. These structures indicate substantial flexibility betweenV1and Voin a working enzyme, which results from mechanical competition between centralshaft rotation and resistance from the peripheral stalks. We also describedetails of adenosine diphosphate inhibition release, V1-Votorque transmission, andproton translocation, which are relevant for the entire V-type ATPase family.
Publishing Year
Date Published
2019-08-23
Journal Title
Science
Acknowledged SSUs
Volume
365
Issue
6455
Article Number
eaaw9144
ISSN
eISSN
IST-REx-ID

Cite this

Zhou L, Sazanov LA. Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase. Science. 2019;365(6455):eaaw9144. doi:10.1126/science.aaw9144
Zhou, L., & Sazanov, L. A. (2019). Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase. Science, 365(6455), eaaw9144. https://doi.org/10.1126/science.aaw9144
Zhou, Long, and Leonid A Sazanov. “Structure and Conformational Plasticity of the Intact Thermus Thermophilus V/A-Type ATPase.” Science 365, no. 6455 (2019): eaaw9144. https://doi.org/10.1126/science.aaw9144.
L. Zhou and L. A. Sazanov, “Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase,” Science, vol. 365, no. 6455, p. eaaw9144, 2019.
Zhou L, Sazanov LA. 2019. Structure and conformational plasticity of the intact Thermus thermophilus V/A-type ATPase. Science. 365(6455), eaaw9144.
Zhou, Long, and Leonid A. Sazanov. “Structure and Conformational Plasticity of the Intact Thermus Thermophilus V/A-Type ATPase.” Science, vol. 365, no. 6455, AAAS, 2019, p. eaaw9144, doi:10.1126/science.aaw9144.
External material:
Popular Science
Description
News on IST Website

Export

Marked Publications

Open Data IST Research Explorer

Sources

PMID: 31439765
PubMed | Europe PMC

Search this title in

Google Scholar