--- res: bibo_abstract: - The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation in vitro, we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation in vivo. We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST in vivo, and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not in vivo. Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Gabriel foaf_name: Krens, Gabriel foaf_surname: Krens foaf_workInfoHomepage: http://www.librecat.org/personId=2B819732-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-4761-5996 - foaf_Person: foaf_givenName: Jim foaf_name: Veldhuis, Jim foaf_surname: Veldhuis - foaf_Person: foaf_givenName: Vanessa foaf_name: Barone, Vanessa foaf_surname: Barone foaf_workInfoHomepage: http://www.librecat.org/personId=419EECCC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-2676-3367 - foaf_Person: foaf_givenName: Daniel foaf_name: Capek, Daniel foaf_surname: Capek foaf_workInfoHomepage: http://www.librecat.org/personId=31C42484-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-5199-9940 - foaf_Person: foaf_givenName: Jean-Léon foaf_name: Maître, Jean-Léon foaf_surname: Maître foaf_workInfoHomepage: http://www.librecat.org/personId=48F1E0D8-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-3688-1474 - foaf_Person: foaf_givenName: Wayne foaf_name: Brodland, Wayne foaf_surname: Brodland - foaf_Person: foaf_givenName: Carl-Philipp J foaf_name: Heisenberg, Carl-Philipp J foaf_surname: Heisenberg foaf_workInfoHomepage: http://www.librecat.org/personId=39427864-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-0912-4566 bibo_doi: 10.1242/dev.144964 bibo_issue: '10' bibo_volume: 144 dct_date: 2017^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/09501991 dct_language: eng dct_publisher: Company of Biologists@ dct_title: Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation@ fabio_hasPubmedId: '28512197' ...