Noisy response to antibiotic stress predicts subsequent single cell survival in an acidic environment

K. Mitosch, G. Rieckh, T. Bollenbach, Cell Systems 4 (2017) 393–403.

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Journal Article | Published | English
Abstract
Antibiotics elicit drastic changes in microbial gene expression, including the induction of stress response genes. While certain stress responses are known to “cross-protect” bacteria from other stressors, it is unclear whether cellular responses to antibiotics have a similar protective role. By measuring the genome-wide transcriptional response dynamics of Escherichia coli to four antibiotics, we found that trimethoprim induces a rapid acid stress response that protects bacteria from subsequent exposure to acid. Combining microfluidics with time-lapse imaging to monitor survival and acid stress response in single cells revealed that the noisy expression of the acid resistance operon gadBC correlates with single-cell survival. Cells with higher gadBC expression following trimethoprim maintain higher intracellular pH and survive the acid stress longer. The seemingly random single-cell survival under acid stress can therefore be predicted from gadBC expression and rationalized in terms of GadB/C molecular function. Overall, we provide a roadmap for identifying the molecular mechanisms of single-cell cross-protection between antibiotics and other stressors.
Publishing Year
Date Published
2017-04-26
Journal Title
Cell Systems
Volume
4
Issue
4
Page
393 - 403
ISSN
IST-REx-ID

Cite this

Mitosch K, Rieckh G, Bollenbach T. Noisy response to antibiotic stress predicts subsequent single cell survival in an acidic environment. Cell Systems. 2017;4(4):393-403. doi:10.1016/j.cels.2017.03.001
Mitosch, K., Rieckh, G., & Bollenbach, T. (2017). Noisy response to antibiotic stress predicts subsequent single cell survival in an acidic environment. Cell Systems, 4(4), 393–403. https://doi.org/10.1016/j.cels.2017.03.001
Mitosch, Karin, Georg Rieckh, and Tobias Bollenbach. “Noisy Response to Antibiotic Stress Predicts Subsequent Single Cell Survival in an Acidic Environment.” Cell Systems 4, no. 4 (2017): 393–403. https://doi.org/10.1016/j.cels.2017.03.001.
K. Mitosch, G. Rieckh, and T. Bollenbach, “Noisy response to antibiotic stress predicts subsequent single cell survival in an acidic environment,” Cell Systems, vol. 4, no. 4, pp. 393–403, 2017.
Mitosch K, Rieckh G, Bollenbach T. 2017. Noisy response to antibiotic stress predicts subsequent single cell survival in an acidic environment. Cell Systems. 4(4), 393–403.
Mitosch, Karin, et al. “Noisy Response to Antibiotic Stress Predicts Subsequent Single Cell Survival in an Acidic Environment.” Cell Systems, vol. 4, no. 4, Cell Press, 2017, pp. 393–403, doi:10.1016/j.cels.2017.03.001.
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