--- res: bibo_abstract: - Growing microtubules are protected from depolymerization by the presence of a GTP or GDP/Pi cap. End-binding proteins of the EB1 family bind to the stabilizing cap, allowing monitoring of its size in real time. The cap size has been shown to correlate with instantaneous microtubule stability. Here we have quantitatively characterized the properties of cap size fluctuations during steadystate growth and have developed a theory predicting their timescale and amplitude from the kinetics of microtubule growth and cap maturation. In contrast to growth speed fluctuations, cap size fluctuations show a characteristic timescale, which is defined by the lifetime of the cap sites. Growth fluctuations affect the amplitude of cap size fluctuations; however, cap size does not affect growth speed, indicating that microtubules are far from instability during most of their time of growth. Our theory provides the basis for a quantitative understanding of microtubule stability fluctuations during steady-state growth.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Jamie foaf_name: Rickman, Jamie foaf_surname: Rickman - foaf_Person: foaf_givenName: Christian F foaf_name: Düllberg, Christian F foaf_surname: Düllberg foaf_workInfoHomepage: http://www.librecat.org/personId=459064DC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-6335-9748 - foaf_Person: foaf_givenName: Nicholas foaf_name: Cade, Nicholas foaf_surname: Cade - foaf_Person: foaf_givenName: Lewis foaf_name: Griffin, Lewis foaf_surname: Griffin - foaf_Person: foaf_givenName: Thomas foaf_name: Surrey, Thomas foaf_surname: Surrey bibo_doi: 10.1073/pnas.1620274114 bibo_issue: '13' bibo_volume: 114 dct_date: 2017^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/00278424 dct_language: eng dct_publisher: National Academy of Sciences@ dct_title: Steady state EB cap size fluctuations are determined by stochastic microtubule growth and maturation@ fabio_hasPubmedId: '28280102' ...