Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges

Maes, Margaret E; Colombo, Gloria; Schulz, Rouven; Siegert, Sandra

Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges

M.E. Maes, G. Colombo, R. Schulz, S. Siegert, Neuroscience Letters 707 (2019).

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DOI

10.1016/j.neulet.2019.134310

Journal Article | Epub ahead of print | English

Author

Maes, Margaret E^{IST Austria} ; Colombo, Gloria^{IST Austria} ; Schulz, Rouven^{IST Austria} ; Siegert, Sandra^{IST Austria}

Department

Siegert Group

Grant

International IST Doctoral Program
Microglia action towards neuronal circuit formation and function in health and disease
Modulating microglia through G protein-coupled receptor (GPCR) signaling

Abstract

Microglia have emerged as a critical component of neurodegenerative diseases. Genetic manipulation of microglia can elucidate their functional impact in disease. In neuroscience, recombinant viruses such as lentiviruses and adeno-associated viruses (AAVs) have been successfully used to target various cell types in the brain, although effective transduction of microglia is rare. In this review, we provide a short background of lentiviruses and AAVs, and strategies for designing recombinant viral vectors. Then, we will summarize recent literature on successful microglial transductions in vitro and in vivo, and discuss the current challenges. Finally, we provide guidelines for reporting the efficiency and specificity of viral targeting in microglia, which will enable the microglial research community to assess and improve methodologies for future studies.

Publishing Year

2019

Date Published

2019-08-10

Journal Title

Neuroscience Letters

Volume

707

Article Number

134310

ISSN

0304-3940

IST-REx-ID

6521

Cite this

Maes ME, Colombo G, Schulz R, Siegert S. Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges. Neuroscience Letters. 2019;707. doi:10.1016/j.neulet.2019.134310

Maes, M. E., Colombo, G., Schulz, R., & Siegert, S. (2019). Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges. Neuroscience Letters, 707. https://doi.org/10.1016/j.neulet.2019.134310

Maes, Margaret E, Gloria Colombo, Rouven Schulz, and Sandra Siegert. “Targeting Microglia with Lentivirus and AAV: Recent Advances and Remaining Challenges.” Neuroscience Letters 707 (2019). https://doi.org/10.1016/j.neulet.2019.134310.

M. E. Maes, G. Colombo, R. Schulz, and S. Siegert, “Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges,” Neuroscience Letters, vol. 707, 2019.

Maes ME, Colombo G, Schulz R, Siegert S. 2019. Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges. Neuroscience Letters. 707.

Maes, Margaret E., et al. “Targeting Microglia with Lentivirus and AAV: Recent Advances and Remaining Challenges.” Neuroscience Letters, vol. 707, 134310, Elsevier, 2019, doi:10.1016/j.neulet.2019.134310.

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