{"language":[{"iso":"eng"}],"author":[{"first_name":"Hélène","full_name":"Chassin, Hélène","last_name":"Chassin"},{"first_name":"Marius","full_name":"Müller, Marius","last_name":"Müller"},{"last_name":"Tigges","full_name":"Tigges, Marcel","first_name":"Marcel"},{"first_name":"Leo","last_name":"Scheller","full_name":"Scheller, Leo"},{"id":"29E0800A-F248-11E8-B48F-1D18A9856A87","last_name":"Lang","full_name":"Lang, Moritz","first_name":"Moritz"},{"full_name":"Fussenegger, Martin","last_name":"Fussenegger","first_name":"Martin"}],"year":"2019","issue":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)"},"day":"01","month":"05","department":[{"_id":"CaGu"}],"file_date_updated":"2020-07-14T12:47:31Z","oa":1,"file":[{"file_name":"2019_NatureComm_Chassin.pdf","relation":"main_file","date_updated":"2020-07-14T12:47:31Z","content_type":"application/pdf","checksum":"e214d3e4f8c81e35981583c4569b51b8","date_created":"2019-05-20T07:33:54Z","access_level":"open_access","file_id":"6471","file_size":1191827,"creator":"dernst"}],"date_created":"2019-05-19T21:59:14Z","external_id":{"isi":["000466338600006"]},"publisher":"Springer Nature","citation":{"apa":"Chassin, H., Müller, M., Tigges, M., Scheller, L., Lang, M., &#38; Fussenegger, M. (2019). A modular degron library for synthetic circuits in mammalian cells. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-019-09974-5\">https://doi.org/10.1038/s41467-019-09974-5</a>","short":"H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, M. Fussenegger, Nature Communications 10 (2019).","chicago":"Chassin, Hélène, Marius Müller, Marcel Tigges, Leo Scheller, Moritz Lang, and Martin Fussenegger. “A Modular Degron Library for Synthetic Circuits in Mammalian Cells.” <i>Nature Communications</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41467-019-09974-5\">https://doi.org/10.1038/s41467-019-09974-5</a>.","ista":"Chassin H, Müller M, Tigges M, Scheller L, Lang M, Fussenegger M. 2019. A modular degron library for synthetic circuits in mammalian cells. Nature Communications. 10(1), 2013.","ama":"Chassin H, Müller M, Tigges M, Scheller L, Lang M, Fussenegger M. A modular degron library for synthetic circuits in mammalian cells. <i>Nature Communications</i>. 2019;10(1). doi:<a href=\"https://doi.org/10.1038/s41467-019-09974-5\">10.1038/s41467-019-09974-5</a>","mla":"Chassin, Hélène, et al. “A Modular Degron Library for Synthetic Circuits in Mammalian Cells.” <i>Nature Communications</i>, vol. 10, no. 1, 2013, Springer Nature, 2019, doi:<a href=\"https://doi.org/10.1038/s41467-019-09974-5\">10.1038/s41467-019-09974-5</a>.","ieee":"H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, and M. Fussenegger, “A modular degron library for synthetic circuits in mammalian cells,” <i>Nature Communications</i>, vol. 10, no. 1. Springer Nature, 2019."},"oa_version":"Published Version","abstract":[{"lang":"eng","text":"Tight control over protein degradation is a fundamental requirement for cells to respond rapidly to various stimuli and adapt to a fluctuating environment. Here we develop a versatile, easy-to-handle library of destabilizing tags (degrons) for the precise regulation of protein expression profiles in mammalian cells by modulating target protein half-lives in a predictable manner. Using the well-established tetracycline gene-regulation system as a model, we show that the dynamics of protein expression can be tuned by fusing appropriate degron tags to gene regulators. Next, we apply this degron library to tune a synthetic pulse-generating circuit in mammalian cells. With this toolbox we establish a set of pulse generators with tailored pulse lengths and magnitudes of protein expression. This methodology will prove useful in the functional roles of essential proteins, fine-tuning of gene-expression systems, and enabling a higher complexity in the design of synthetic biological systems in mammalian cells."}],"publication":"Nature Communications","publication_identifier":{"eissn":["20411723"]},"publication_status":"published","ddc":["570"],"date_updated":"2023-08-25T10:33:51Z","article_number":"2013","status":"public","isi":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","related_material":{"link":[{"relation":"erratum","url":"https://doi.org/10.1038/s41467-023-36111-0"}]},"intvolume":"        10","scopus_import":"1","has_accepted_license":"1","_id":"6465","doi":"10.1038/s41467-019-09974-5","article_processing_charge":"No","date_published":"2019-05-01T00:00:00Z","title":"A modular degron library for synthetic circuits in mammalian cells","quality_controlled":"1","volume":10,"type":"journal_article"}