---
_id: '6124'
abstract:
- lang: eng
text: Despite the importance of G-protein coupled receptors (GPCRs) their biogenesis
is poorly understood. Like vertebrates, C. elegans uses a large family of GPCRs
as chemoreceptors. A subset of these receptors, such as ODR-10, requires the odr-4
and odr-8 genes to be appropriately localized to sensory cilia. The odr-4 gene
encodes a conserved tail-anchored transmembrane protein; the molecular identity
of odr-8 is unknown. Here, we show that odr-8 encodes the C. elegans ortholog
of Ufm1-specific protease 2 (UfSP2). UfSPs are cysteine proteases identified biochemically
by their ability to liberate the ubiquitin-like modifier Ufm1 from its pro-form
and protein conjugates. ODR-8/UfSP2 and ODR-4 are expressed in the same set of
twelve chemosensory neurons, and physically interact at the ER membrane. ODR-4
also binds ODR-10, suggesting that an ODR-4/ODR-8 complex promotes GPCR folding,
maturation, or export from the ER. The physical interaction between human ODR4
and UfSP2 suggests that this complex's role in GPCR biogenesis may be evolutionarily
conserved. Unexpectedly, mutant versions of ODR-8/UfSP2 lacking catalytic residues
required for protease activity can rescue all odr-8 mutant phenotypes tested.
Moreover, deleting C. elegans ufm-1 does not alter chemoreceptor traffic to cilia,
either in wild type or in odr-8 mutants. Thus, UfSP2 proteins have protease- and
Ufm1-independent functions in GPCR biogenesis.
article_number: e1004082
author:
- first_name: Changchun
full_name: Chen, Changchun
last_name: Chen
- first_name: Eisuke
full_name: Itakura, Eisuke
last_name: Itakura
- first_name: Katherine P.
full_name: Weber, Katherine P.
last_name: Weber
- first_name: Ramanujan S.
full_name: Hegde, Ramanujan S.
last_name: Hegde
- first_name: Mario
full_name: de Bono, Mario
id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
last_name: de Bono
orcid: 0000-0001-8347-0443
citation:
ama: Chen C, Itakura E, Weber KP, Hegde RS, de Bono M. An ER complex of ODR-4 and
ODR-8/Ufm1 specific protease 2 promotes GPCR maturation by a Ufm1-independent
mechanism. PLoS Genetics. 2014;10(3). doi:10.1371/journal.pgen.1004082
apa: Chen, C., Itakura, E., Weber, K. P., Hegde, R. S., & de Bono, M. (2014).
An ER complex of ODR-4 and ODR-8/Ufm1 specific protease 2 promotes GPCR maturation
by a Ufm1-independent mechanism. PLoS Genetics. Public Library of Science
(PLoS). https://doi.org/10.1371/journal.pgen.1004082
chicago: Chen, Changchun, Eisuke Itakura, Katherine P. Weber, Ramanujan S. Hegde,
and Mario de Bono. “An ER Complex of ODR-4 and ODR-8/Ufm1 Specific Protease 2
Promotes GPCR Maturation by a Ufm1-Independent Mechanism.” PLoS Genetics.
Public Library of Science (PLoS), 2014. https://doi.org/10.1371/journal.pgen.1004082.
ieee: C. Chen, E. Itakura, K. P. Weber, R. S. Hegde, and M. de Bono, “An ER complex
of ODR-4 and ODR-8/Ufm1 specific protease 2 promotes GPCR maturation by a Ufm1-independent
mechanism,” PLoS Genetics, vol. 10, no. 3. Public Library of Science (PLoS),
2014.
ista: Chen C, Itakura E, Weber KP, Hegde RS, de Bono M. 2014. An ER complex of ODR-4
and ODR-8/Ufm1 specific protease 2 promotes GPCR maturation by a Ufm1-independent
mechanism. PLoS Genetics. 10(3), e1004082.
mla: Chen, Changchun, et al. “An ER Complex of ODR-4 and ODR-8/Ufm1 Specific Protease
2 Promotes GPCR Maturation by a Ufm1-Independent Mechanism.” PLoS Genetics,
vol. 10, no. 3, e1004082, Public Library of Science (PLoS), 2014, doi:10.1371/journal.pgen.1004082.
short: C. Chen, E. Itakura, K.P. Weber, R.S. Hegde, M. de Bono, PLoS Genetics 10
(2014).
date_created: 2019-03-19T14:45:56Z
date_published: 2014-03-06T00:00:00Z
date_updated: 2021-01-12T08:06:14Z
day: '06'
ddc:
- '570'
doi: 10.1371/journal.pgen.1004082
extern: '1'
external_id:
pmid:
- '24603482'
file:
- access_level: open_access
checksum: ac19941089a4262bb5bd74434a08b003
content_type: application/pdf
creator: kschuh
date_created: 2019-03-19T14:50:07Z
date_updated: 2020-07-14T12:47:20Z
file_id: '6125'
file_name: 2014_PLOS_Chen.PDF
file_size: 8286819
relation: main_file
file_date_updated: 2020-07-14T12:47:20Z
has_accepted_license: '1'
intvolume: ' 10'
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLoS Genetics
publication_identifier:
issn:
- 1553-7404
publication_status: published
publisher: Public Library of Science (PLoS)
quality_controlled: '1'
status: public
title: An ER complex of ODR-4 and ODR-8/Ufm1 specific protease 2 promotes GPCR maturation
by a Ufm1-independent mechanism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 10
year: '2014'
...