Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp

C.A. Bernecky, J. Plitzko, P. Cramer, Nature Structural and Molecular Biology 24 (2017) 809–815.

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Journal Article | Published | English
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Abstract
During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography to determine the structure of the mammalian Pol II-DSIF elongation complex at a nominal resolution of 3.4. Human DSIF has a modular structure with two domains forming a DNA clamp, two domains forming an RNA clamp, and one domain buttressing the RNA clamp. The clamps maintain the transcription bubble, position upstream DNA, and retain the RNA transcript in the exit tunnel. The mobile C-terminal region of DSIF is located near exiting RNA, where it can recruit factors for RNA processing. The structure provides insight into the roles of DSIF during mRNA synthesis.
Publishing Year
Date Published
2017-10-05
Journal Title
Nature Structural and Molecular Biology
Volume
24
Issue
10
Page
809 - 815
ISSN
IST-REx-ID

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Bernecky CA, Plitzko J, Cramer P. Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp. Nature Structural and Molecular Biology. 2017;24(10):809-815. doi:10.1038/nsmb.3465
Bernecky, C. A., Plitzko, J., & Cramer, P. (2017). Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp. Nature Structural and Molecular Biology, 24(10), 809–815. https://doi.org/10.1038/nsmb.3465
Bernecky, Carrie A, Jürgen Plitzko, and Patrick Cramer. “Structure of a Transcribing RNA Polymerase II-DSIF Complex Reveals a Multidentate DNA-RNA Clamp.” Nature Structural and Molecular Biology 24, no. 10 (2017): 809–15. https://doi.org/10.1038/nsmb.3465.
C. A. Bernecky, J. Plitzko, and P. Cramer, “Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp,” Nature Structural and Molecular Biology, vol. 24, no. 10, pp. 809–815, 2017.
Bernecky CA, Plitzko J, Cramer P. 2017. Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp. Nature Structural and Molecular Biology. 24(10), 809–815.
Bernecky, Carrie A., et al. “Structure of a Transcribing RNA Polymerase II-DSIF Complex Reveals a Multidentate DNA-RNA Clamp.” Nature Structural and Molecular Biology, vol. 24, no. 10, Nature Publishing Group, 2017, pp. 809–15, doi:10.1038/nsmb.3465.

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