Structural basis of RNA polymerase I transcription initiation

C. Engel, T. Gubbey, S. Neyer, S. Sainsbury, C. Oberthuer, C. Baejen, C.A. Bernecky, P. Cramer, Cell 169 (2017) 120–131.e22.

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Journal Article | Published | English
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Abstract
Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct “bendability” and “meltability” of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase.
Publishing Year
Date Published
2017-03-23
Journal Title
Cell
Volume
169
Issue
1
Page
120 - 131.e22
ISSN
IST-REx-ID

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Engel C, Gubbey T, Neyer S, et al. Structural basis of RNA polymerase I transcription initiation. Cell. 2017;169(1):120-131.e22. doi:10.1016/j.cell.2017.03.003
Engel, C., Gubbey, T., Neyer, S., Sainsbury, S., Oberthuer, C., Baejen, C., … Cramer, P. (2017). Structural basis of RNA polymerase I transcription initiation. Cell, 169(1), 120–131.e22. https://doi.org/10.1016/j.cell.2017.03.003
Engel, Christoph, Tobias Gubbey, Simon Neyer, Sarah Sainsbury, Christiane Oberthuer, Carlo Baejen, Carrie A Bernecky, and Patrick Cramer. “Structural Basis of RNA Polymerase I Transcription Initiation.” Cell 169, no. 1 (2017): 120–131.e22. https://doi.org/10.1016/j.cell.2017.03.003.
C. Engel et al., “Structural basis of RNA polymerase I transcription initiation,” Cell, vol. 169, no. 1, p. 120–131.e22, 2017.
Engel C, Gubbey T, Neyer S, Sainsbury S, Oberthuer C, Baejen C, Bernecky CA, Cramer P. 2017. Structural basis of RNA polymerase I transcription initiation. Cell. 169(1), 120–131.e22.
Engel, Christoph, et al. “Structural Basis of RNA Polymerase I Transcription Initiation.” Cell, vol. 169, no. 1, Cell Press, 2017, p. 120–131.e22, doi:10.1016/j.cell.2017.03.003.

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