--- res: bibo_abstract: - CLE peptides have been implicated in various developmental processes of plants and mediate their responses to environmental stimuli. However, the biological relevance of most CLE genes remains to be functionally characterized. Here, we report that CLE9, which is expressed in stomata, acts as an essential regulator in the induction of stomatal closure. Exogenous application of CLE9 peptides or overexpression of CLE9 effectively led to stomatal closure and enhanced drought tolerance, whereas CLE9 loss-of-function mutants were sensitivity to drought stress. CLE9-induced stomatal closure was impaired in abscisic acid (ABA)-deficient mutants, indicating that ABA is required for CLE9-medaited guard cell signalling. We further deciphered that two guard cell ABA-signalling components, OST1 and SLAC1, were responsible for CLE9-induced stomatal closure. MPK3 and MPK6 were activated by the CLE9 peptide, and CLE9 peptides failed to close stomata in mpk3 and mpk6 mutants. In addition, CLE9 peptides stimulated the induction of hydrogen peroxide (H2O2) and nitric oxide (NO) synthesis associated with stomatal closure, which was abolished in the NADPH oxidase-deficient mutants or nitric reductase mutants, respectively. Collectively, our results reveal a novel ABA-dependent function of CLE9 in the regulation of stomatal apertures, thereby suggesting a potential role of CLE9 in the stress acclimatization of plants.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Luosha foaf_name: Zhang, Luosha foaf_surname: Zhang - foaf_Person: foaf_givenName: Xiong foaf_name: Shi, Xiong foaf_surname: Shi - foaf_Person: foaf_givenName: Yutao foaf_name: Zhang, Yutao foaf_surname: Zhang - foaf_Person: foaf_givenName: Jiajing foaf_name: Wang, Jiajing foaf_surname: Wang - foaf_Person: foaf_givenName: Jingwei foaf_name: Yang, Jingwei foaf_surname: Yang - foaf_Person: foaf_givenName: Takashi foaf_name: Ishida, Takashi foaf_surname: Ishida - foaf_Person: foaf_givenName: Wenqian foaf_name: Jiang, Wenqian foaf_surname: Jiang - foaf_Person: foaf_givenName: Xiangyu foaf_name: Han, Xiangyu foaf_surname: Han - foaf_Person: foaf_givenName: Jingke foaf_name: Kang, Jingke foaf_surname: Kang - foaf_Person: foaf_givenName: Xuening foaf_name: Wang, Xuening foaf_surname: Wang - foaf_Person: foaf_givenName: Lixia foaf_name: Pan, Lixia foaf_surname: Pan - foaf_Person: foaf_givenName: Shuo foaf_name: Lv, Shuo foaf_surname: Lv - foaf_Person: foaf_givenName: Bing foaf_name: Cao, Bing foaf_surname: Cao - foaf_Person: foaf_givenName: Yonghong foaf_name: Zhang, Yonghong foaf_surname: Zhang - foaf_Person: foaf_givenName: Jinbin foaf_name: Wu, Jinbin foaf_surname: Wu - foaf_Person: foaf_givenName: Huibin foaf_name: Han, Huibin foaf_surname: Han foaf_workInfoHomepage: http://www.librecat.org/personId=31435098-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Zhubing foaf_name: Hu, Zhubing foaf_surname: Hu - foaf_Person: foaf_givenName: Langjun foaf_name: Cui, Langjun foaf_surname: Cui - foaf_Person: foaf_givenName: Shinichiro foaf_name: Sawa, Shinichiro foaf_surname: Sawa - foaf_Person: foaf_givenName: Junmin foaf_name: He, Junmin foaf_surname: He - foaf_Person: foaf_givenName: Guodong foaf_name: Wang, Guodong foaf_surname: Wang bibo_doi: 10.1111/pce.13475 dct_date: 2018^xs_gYear dct_identifier: - UT:000459014800021 dct_isPartOf: - http://id.crossref.org/issn/01407791 dct_language: eng dct_publisher: Wiley@ dct_title: CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen peroxide, and nitric oxide in arabidopsis thaliana@ fabio_hasPubmedId: '30378140' ...