Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments

F. Spira, S. Cuylen Haering, S. Mehta, M. Samwer, A. REVERSAT, A. Verma, R. Oldenbourg, M.K. Sixt, D. Gerlich, ELife 6 (2017).

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Journal Article | Published | English

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Author
Spira, Felix; Cuylen Haering, Sara; Mehta, Shalin; Samwer, Matthias; REVERSAT, AnneIST Austria ; Verma, Amitabh; Oldenbourg, Rudolf; Sixt, Michael KIST Austria ; Gerlich, Daniel
Department
Abstract
The actomyosin ring generates force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organization and the mechanism of contractility is not well understood. We quantified actin filament order in human cells using fluorescence polarization microscopy and found that cleavage furrow ingression initiates by contraction of an equatorial actin network with randomly oriented filaments. The network subsequently gradually reoriented actin filaments along the cell equator. This strictly depended on myosin II activity, suggesting local network reorganization by mechanical forces. Cortical laser microsurgery revealed that during cytokinesis progression, mechanical tension increased substantially along the direction of the cell equator, while the network contracted laterally along the pole-to-pole axis without a detectable increase in tension. Our data suggest that an asymmetric increase in cortical tension promotes filament reorientation along the cytokinetic cleavage furrow, which might have implications for diverse other biological processes involving actomyosin rings.
Publishing Year
Date Published
2017-11-06
Journal Title
eLife
Volume
6
Article Number
e30867
ISSN
IST-REx-ID
569

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Spira F, Cuylen Haering S, Mehta S, et al. Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments. eLife. 2017;6. doi:10.7554/eLife.30867
Spira, F., Cuylen Haering, S., Mehta, S., Samwer, M., REVERSAT, A., Verma, A., … Gerlich, D. (2017). Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments. ELife, 6. https://doi.org/10.7554/eLife.30867
Spira, Felix, Sara Cuylen Haering, Shalin Mehta, Matthias Samwer, Anne REVERSAT, Amitabh Verma, Rudolf Oldenbourg, Michael K Sixt, and Daniel Gerlich. “Cytokinesis in Vertebrate Cells Initiates by Contraction of an Equatorial Actomyosin Network Composed of Randomly Oriented Filaments.” ELife 6 (2017). https://doi.org/10.7554/eLife.30867.
F. Spira et al., “Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments,” eLife, vol. 6, 2017.
Spira F, Cuylen Haering S, Mehta S, Samwer M, REVERSAT A, Verma A, Oldenbourg R, Sixt MK, Gerlich D. 2017. Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments. eLife. 6.
Spira, Felix, et al. “Cytokinesis in Vertebrate Cells Initiates by Contraction of an Equatorial Actomyosin Network Composed of Randomly Oriented Filaments.” ELife, vol. 6, e30867, eLife Sciences Publications, 2017, doi:10.7554/eLife.30867.
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2018-12-12
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