Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel

D. von Wangenheim, R. Hauschild, J. Friml, Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel, IST Austria, 2017.

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OA IST-2017-66-v1+1_WangenheimHighResolution55044-NEW_1.mp4 101.50 MB

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Abstract
One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. The Video is licensed under a CC BY NC ND license.
Publishing Year
Date Published
2017-04-10
Acknowledgement
fund: FP7-ERC 0101109
IST-REx-ID

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von Wangenheim D, Hauschild R, Friml J. Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel. IST Austria; 2017. doi:10.15479/AT:ISTA:66
von Wangenheim, D., Hauschild, R., & Friml, J. (2017). Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. IST Austria. https://doi.org/10.15479/AT:ISTA:66
Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel. IST Austria, 2017. https://doi.org/10.15479/AT:ISTA:66.
D. von Wangenheim, R. Hauschild, and J. Friml, Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. IST Austria, 2017.
von Wangenheim D, Hauschild R, Friml J. 2017. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel, IST Austria,p.
von Wangenheim, Daniel, et al. Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel. IST Austria, 2017, doi:10.15479/AT:ISTA:66.
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2018-12-12
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