{"publication_status":"published","title":"Cell-to-cell variation and specialization in sugar metabolism in clonal bacterial populations","publisher":"Public Library of Science","intvolume":"        13","quality_controlled":"1","abstract":[{"lang":"eng","text":"While we have good understanding of bacterial metabolism at the population level, we know little about the metabolic behavior of individual cells: do single cells in clonal populations sometimes specialize on different metabolic pathways? Such metabolic specialization could be driven by stochastic gene expression and could provide individual cells with growth benefits of specialization. We measured the degree of phenotypic specialization in two parallel metabolic pathways, the assimilation of glucose and arabinose. We grew Escherichia coli in chemostats, and used isotope-labeled sugars in combination with nanometer-scale secondary ion mass spectrometry and mathematical modeling to quantify sugar assimilation at the single-cell level. We found large variation in metabolic activities between single cells, both in absolute assimilation and in the degree to which individual cells specialize in the assimilation of different sugars. Analysis of transcriptional reporters indicated that this variation was at least partially based on cell-to-cell variation in gene expression. Metabolic differences between cells in clonal populations could potentially reduce metabolic incompatibilities between different pathways, and increase the rate at which parallel reactions can be performed."}],"date_updated":"2023-02-23T14:10:34Z","ec_funded":1,"language":[{"iso":"eng"}],"date_published":"2017-12-18T00:00:00Z","date_created":"2018-12-11T11:47:04Z","issue":"12","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2017","oa_version":"Published Version","related_material":{"record":[{"status":"public","relation":"research_data","id":"9844"},{"id":"9845","status":"public","relation":"research_data"},{"relation":"research_data","status":"public","id":"9846"}]},"month":"12","publication_identifier":{"issn":["15537390"]},"author":[{"orcid":"0000-0001-9068-6090","last_name":"Nikolic","id":"42D9CABC-F248-11E8-B48F-1D18A9856A87","first_name":"Nela","full_name":"Nikolic, Nela"},{"last_name":"Schreiber","full_name":"Schreiber, Frank","first_name":"Frank"},{"first_name":"Alma","full_name":"Dal Co, Alma","last_name":"Dal Co"},{"first_name":"Daniel","full_name":"Kiviet, Daniel","last_name":"Kiviet"},{"first_name":"Tobias","full_name":"Bergmiller, Tobias","last_name":"Bergmiller","orcid":"0000-0001-5396-4346","id":"2C471CFA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Sten","full_name":"Littmann, Sten","last_name":"Littmann"},{"last_name":"Kuypers","full_name":"Kuypers, Marcel","first_name":"Marcel"},{"full_name":"Ackermann, Martin","first_name":"Martin","last_name":"Ackermann"}],"pubrep_id":"959","day":"18","doi":"10.1371/journal.pgen.1007122","_id":"541","file_date_updated":"2020-07-14T12:46:46Z","oa":1,"file":[{"creator":"system","relation":"main_file","date_updated":"2020-07-14T12:46:46Z","access_level":"open_access","file_name":"IST-2018-959-v1+1_2017_Nikolic_Cell-to-cell.pdf","file_id":"5088","checksum":"22426d9382f21554bad5fa5967afcfd0","date_created":"2018-12-12T10:14:35Z","content_type":"application/pdf","file_size":1308475}],"project":[{"grant_number":"291734","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme"}],"publication":"PLoS Genetics","volume":13,"scopus_import":1,"citation":{"apa":"Nikolic, N., Schreiber, F., Dal Co, A., Kiviet, D., Bergmiller, T., Littmann, S., … Ackermann, M. (2017). Cell-to-cell variation and specialization in sugar metabolism in clonal bacterial populations. <i>PLoS Genetics</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pgen.1007122\">https://doi.org/10.1371/journal.pgen.1007122</a>","chicago":"Nikolic, Nela, Frank Schreiber, Alma Dal Co, Daniel Kiviet, Tobias Bergmiller, Sten Littmann, Marcel Kuypers, and Martin Ackermann. “Cell-to-Cell Variation and Specialization in Sugar Metabolism in Clonal Bacterial Populations.” <i>PLoS Genetics</i>. Public Library of Science, 2017. <a href=\"https://doi.org/10.1371/journal.pgen.1007122\">https://doi.org/10.1371/journal.pgen.1007122</a>.","short":"N. Nikolic, F. Schreiber, A. Dal Co, D. Kiviet, T. Bergmiller, S. Littmann, M. Kuypers, M. Ackermann, PLoS Genetics 13 (2017).","ieee":"N. Nikolic <i>et al.</i>, “Cell-to-cell variation and specialization in sugar metabolism in clonal bacterial populations,” <i>PLoS Genetics</i>, vol. 13, no. 12. Public Library of Science, 2017.","mla":"Nikolic, Nela, et al. “Cell-to-Cell Variation and Specialization in Sugar Metabolism in Clonal Bacterial Populations.” <i>PLoS Genetics</i>, vol. 13, no. 12, e1007122, Public Library of Science, 2017, doi:<a href=\"https://doi.org/10.1371/journal.pgen.1007122\">10.1371/journal.pgen.1007122</a>.","ama":"Nikolic N, Schreiber F, Dal Co A, et al. Cell-to-cell variation and specialization in sugar metabolism in clonal bacterial populations. <i>PLoS Genetics</i>. 2017;13(12). doi:<a href=\"https://doi.org/10.1371/journal.pgen.1007122\">10.1371/journal.pgen.1007122</a>","ista":"Nikolic N, Schreiber F, Dal Co A, Kiviet D, Bergmiller T, Littmann S, Kuypers M, Ackermann M. 2017. Cell-to-cell variation and specialization in sugar metabolism in clonal bacterial populations. PLoS Genetics. 13(12), e1007122."},"tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"department":[{"_id":"CaGu"}],"type":"journal_article","publist_id":"7275","ddc":["576","579"],"status":"public","article_number":"e1007122","has_accepted_license":"1"}