--- res: bibo_abstract: - Most kinesin motors move in only one direction along microtubules. Members of the kinesin-5 subfamily were initially described as unidirectional plus-end-directed motors and shown to produce piconewton forces. However, some fungal kinesin-5 motors are bidirectional. The force production of a bidirectional kinesin-5 has not yet been measured. Therefore, it remains unknown whether the mechanism of the unconventional minus-end-directed motility differs fundamentally from that of plus-end-directed stepping. Using force spectroscopy, we have measured here the forces that ensembles of purified budding yeast kinesin-5 Cin8 produce in microtubule gliding assays in both plus- and minus-end direction. Correlation analysis of pause forces demonstrated that individual Cin8 molecules produce additive forces in both directions of movement. In ensembles, Cin8 motors were able to produce single-motor forces up to a magnitude of ∼1.5 pN. Hence, these properties appear to be conserved within the kinesin-5 subfamily. Force production was largely independent of the directionality of movement, indicating similarities between the motility mechanisms for both directions. These results provide constraints for the development of models for the bidirectional motility mechanism of fission yeast kinesin-5 and provide insight into the function of this mitotic motor.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Todd foaf_name: Fallesen, Todd foaf_surname: Fallesen - foaf_Person: foaf_givenName: Johanna foaf_name: Roostalu, Johanna foaf_surname: Roostalu - foaf_Person: foaf_givenName: Christian F foaf_name: Düllberg, Christian F foaf_surname: Düllberg foaf_workInfoHomepage: http://www.librecat.org/personId=459064DC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-6335-9748 - foaf_Person: foaf_givenName: Gunnar foaf_name: Pruessner, Gunnar foaf_surname: Pruessner - foaf_Person: foaf_givenName: Thomas foaf_name: Surrey, Thomas foaf_surname: Surrey bibo_doi: 10.1016/j.bpj.2017.09.006 bibo_issue: '9' bibo_volume: 113 dct_date: 2017^xs_gYear dct_language: eng dct_publisher: Biophysical Society@ dct_title: Ensembles of bidirectional kinesin Cin8 produce additive forces in both directions of movement@ ...