---
_id: '4224'
abstract:
- lang: eng
text: Developing cells acquire positional information by reading the graded distribution
of morphogens. In Drosophila, the Dpp morphogen forms a long-range concentration
gradient by spreading from a restricted source in the developing wing. It has
been assumed that Dpp spreads by extracellular diffusion. Under this assumption,
the main role of endocytosis in gradient formation is to downregulate receptors
at the cell surface. These surface receptors bind to the ligand and thereby interfere
with its long-range movement. Recent experiments indicate that Dpp spreading is
mediated by Dynamin-dependent endocytosis in the target tissue, suggesting that
extracellular diffusion alone cannot account for Dpp dispersal. Here, we perform
a theoretical study of a model for morphogen spreading based on extracellular
diffusion, which takes into account receptor binding and trafficking. We compare
profiles of ligand and surface receptors obtained in this model with experimental
data. To this end, we monitored directly the pool of surface receptors and extracellular
Dpp with specific antibodies. We conclude that current models considering pure
extracellular diffusion cannot explain the observed role of endocytosis during
Dpp long-range movement.
article_processing_charge: No
author:
- first_name: Karsten
full_name: Kruse, Karsten
last_name: Kruse
- first_name: Periklis
full_name: Pantazis, Periklis
last_name: Pantazis
- first_name: Mark Tobias
full_name: Bollenbach, Mark Tobias
id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
last_name: Bollenbach
orcid: 0000-0003-4398-476X
- first_name: Frank
full_name: Julicher, Frank
last_name: Julicher
- first_name: Marcos
full_name: Gonzalez Gaitan, Marcos
last_name: Gonzalez Gaitan
citation:
ama: 'Kruse K, Pantazis P, Bollenbach MT, Julicher F, Gonzalez Gaitan M. Dpp gradient
formation by dynamin-dependent endocytosis: receptor trafficking and the diffusion
model. Development. 2004;131(19):4843-4856. doi:10.1242/dev.01335'
apa: 'Kruse, K., Pantazis, P., Bollenbach, M. T., Julicher, F., & Gonzalez Gaitan,
M. (2004). Dpp gradient formation by dynamin-dependent endocytosis: receptor trafficking
and the diffusion model. Development. Company of Biologists. https://doi.org/10.1242/dev.01335'
chicago: 'Kruse, Karsten, Periklis Pantazis, Mark Tobias Bollenbach, Frank Julicher,
and Marcos Gonzalez Gaitan. “Dpp Gradient Formation by Dynamin-Dependent Endocytosis:
Receptor Trafficking and the Diffusion Model.” Development. Company of
Biologists, 2004. https://doi.org/10.1242/dev.01335.'
ieee: 'K. Kruse, P. Pantazis, M. T. Bollenbach, F. Julicher, and M. Gonzalez Gaitan,
“Dpp gradient formation by dynamin-dependent endocytosis: receptor trafficking
and the diffusion model,” Development, vol. 131, no. 19. Company of Biologists,
pp. 4843–4856, 2004.'
ista: 'Kruse K, Pantazis P, Bollenbach MT, Julicher F, Gonzalez Gaitan M. 2004.
Dpp gradient formation by dynamin-dependent endocytosis: receptor trafficking
and the diffusion model. Development. 131(19), 4843–4856.'
mla: 'Kruse, Karsten, et al. “Dpp Gradient Formation by Dynamin-Dependent Endocytosis:
Receptor Trafficking and the Diffusion Model.” Development, vol. 131, no.
19, Company of Biologists, 2004, pp. 4843–56, doi:10.1242/dev.01335.'
short: K. Kruse, P. Pantazis, M.T. Bollenbach, F. Julicher, M. Gonzalez Gaitan,
Development 131 (2004) 4843–4856.
date_created: 2018-12-11T12:07:41Z
date_published: 2004-01-01T00:00:00Z
date_updated: 2021-01-12T07:55:26Z
day: '01'
doi: 10.1242/dev.01335
extern: '1'
intvolume: ' 131'
issue: '19'
language:
- iso: eng
month: '01'
oa_version: None
page: 4843 - 4856
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '1893'
status: public
title: 'Dpp gradient formation by dynamin-dependent endocytosis: receptor trafficking
and the diffusion model'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 131
year: '2004'
...