{"abstract":[{"text":"Mutations in two genes affect the formation of the boundary between midbrain and hindbrain (MHB): no isthmus (noi) and acerebellar (ace), noi mutant embryos lack the MHB constriction, the cerebellum and optic tectum, as well as the pronephric duct. Analysis of noi mutant embryos with neuron-specific antibodies shows that the MHB region and the dorsal and ventral midbrain are absent or abnormal, but that the rostral hindbrain is unaffected with the exception of the cerebellum, Using markers that are expressed during its formation (eng, wnt1 and pax-b), we find that the MHB region is already misspecified in noi mutant embryos during late gastrulation. The tectum is initially present and later degenerates, The defect in ace mutant embryos is more restricted: MHB and cerebellum are absent, but a tectum is formed, Molecular organisation of the tectum and tegmentum is disturbed, however, since eng, wntl and pax-b marker gene expression is not maintained, We propose that noi and ace are required for development of the MHB region and of the adjacent mid- and hindbrain, which are thought to be patterned by the MHB region, Presence of pax-b RNA, and absence of pax-b protein, together with the observation of genetic linkage and the occurrence of a point mutation, show that noi mutations are located in the pax-b gene, pax-b is a vertebrate orthologue of the Drosophila gene paired, which is involved in a pathway of cellular interactions at the posterior compartment boundary in Drosophila, Our results confirm and extend a previous report, and show that at least one member of this conserved signalling pathway is required for formation of the boundary between midbrain and hindbrain in the zebrafish.","lang":"eng"}],"citation":{"ama":"Brand M, Heisenberg C-PJ, Jiang Y, et al. Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. <i>Development</i>. 1996;123(1):179-190. doi:<a href=\"https://doi.org/10.1242/dev.123.1.179 \">10.1242/dev.123.1.179 </a>","apa":"Brand, M., Heisenberg, C.-P. J., Jiang, Y., Beuchle, D., Lun, K., Furutani Seiki, M., … Nüsslein Volhard, C. (1996). Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.123.1.179 \">https://doi.org/10.1242/dev.123.1.179 </a>","chicago":"Brand, Michael, Carl-Philipp J Heisenberg, Yunjin Jiang, Dirk Beuchle, Klaus Lun, Makoto Furutani Seiki, Michael Granato, et al. “Mutations in Zebrafish Genes Affecting the Formation of the Boundary between Midbrain and Hindbrain.” <i>Development</i>. Company of Biologists, 1996. <a href=\"https://doi.org/10.1242/dev.123.1.179 \">https://doi.org/10.1242/dev.123.1.179 </a>.","ieee":"M. Brand <i>et al.</i>, “Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain,” <i>Development</i>, vol. 123, no. 1. Company of Biologists, pp. 179–190, 1996.","ista":"Brand M, Heisenberg C-PJ, Jiang Y, Beuchle D, Lun K, Furutani Seiki M, Granato M, Haffter P, Hammerschmidt M, Kane D, Kelsh R, Mullins M, Odenthal J, Van Eeden F, Nüsslein Volhard C. 1996. Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. Development. 123(1), 179–190.","short":"M. Brand, C.-P.J. Heisenberg, Y. Jiang, D. Beuchle, K. Lun, M. Furutani Seiki, M. Granato, P. Haffter, M. Hammerschmidt, D. Kane, R. Kelsh, M. Mullins, J. Odenthal, F. Van Eeden, C. Nüsslein Volhard, Development 123 (1996) 179–190.","mla":"Brand, Michael, et al. “Mutations in Zebrafish Genes Affecting the Formation of the Boundary between Midbrain and Hindbrain.” <i>Development</i>, vol. 123, no. 1, Company of Biologists, 1996, pp. 179–90, doi:<a href=\"https://doi.org/10.1242/dev.123.1.179 \">10.1242/dev.123.1.179 </a>."},"language":[{"iso":"eng"}],"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","title":"Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain","author":[{"last_name":"Brand","full_name":"Brand, Michael","first_name":"Michael"},{"last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"},{"full_name":"Jiang, Yunjin","last_name":"Jiang","first_name":"Yunjin"},{"last_name":"Beuchle","full_name":"Beuchle, Dirk","first_name":"Dirk"},{"first_name":"Klaus","last_name":"Lun","full_name":"Lun, Klaus"},{"first_name":"Makoto","last_name":"Furutani Seiki","full_name":"Furutani Seiki, Makoto"},{"full_name":"Granato, Michael","last_name":"Granato","first_name":"Michael"},{"first_name":"Pascal","last_name":"Haffter","full_name":"Haffter, Pascal"},{"first_name":"Matthias","full_name":"Hammerschmidt, Matthias","last_name":"Hammerschmidt"},{"first_name":"Donald","last_name":"Kane","full_name":"Kane, Donald"},{"first_name":"Robert","last_name":"Kelsh","full_name":"Kelsh, Robert"},{"full_name":"Mullins, Mary","last_name":"Mullins","first_name":"Mary"},{"last_name":"Odenthal","full_name":"Odenthal, Jörg","first_name":"Jörg"},{"first_name":"Fredericus","last_name":"Van Eeden","full_name":"Van Eeden, Fredericus"},{"first_name":"Christiane","full_name":"Nüsslein Volhard, Christiane","last_name":"Nüsslein Volhard"}],"publication_status":"published","main_file_link":[{"open_access":"1","url":"https://journals.biologists.com/dev/article/123/1/179/39324/Mutations-in-zebrafish-genes-affecting-the"}],"oa":1,"article_type":"original","issue":"1","acknowledgement":"We would like to thank our colleagues in the zebrafish community for generously sharing antibodies and probes, in particular Terje Johannsen, Vladimir Korzh, Stefan Krauss and Ingvild Mikkola, as well as Christine Dreyer, Nigel Holder, Tom Jessel, Trevor Jowett, Anders Molven, Eric Weinberg and Monte Westerfield. M.B would like to thank his colleagues for numerous discussions, and Francisco Pelegri, Suresh Jesuthasan and Luis Puelles for comments on the\r\nmanuscipt. Thanks also to Peter Andermann and Eric Weinberg, who helped in the analysis of Zash expression, and especially to Corinne Houart, for her lovely in situ protocol and many discussions. Silke Hein helped greatly in final stages of this work. M.B. was supported by a Helmholtz stipend of the BMFT.","external_id":{"pmid":["9007239 "]},"volume":123,"publication":"Development","status":"public","pmid":1,"publist_id":"1899","date_created":"2018-12-11T12:07:40Z","article_processing_charge":"No","doi":"10.1242/dev.123.1.179 ","date_published":"1996-12-01T00:00:00Z","extern":"1","intvolume":"       123","month":"12","day":"01","type":"journal_article","scopus_import":"1","oa_version":"Published Version","date_updated":"2022-08-04T11:45:04Z","quality_controlled":"1","page":"179 - 190","year":"1996","publication_identifier":{"issn":["0950-1991"]},"publisher":"Company of Biologists","_id":"4219"}