A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon
C.-P.J. Heisenberg, C. Houart, M. Take Uchi, G. Rauch, N. Young, P. Coutinho, I. Masai, L. Caneparo, M. Concha, R. Geisler, T. Dale, S. Wilson, D. Stemple, Genes and Development 15 (2001) 1427–1434.
Download
No fulltext has been uploaded. References only!
Journal Article
| Published
| English
Author
Heisenberg, Carl-Philipp IST Austria 
;
Houart, Corinne;
Take Uchi, Masaya;
Rauch, Gerd;
Young, Neville;
Coutinho, Pedro;
Masai, Ichiro;
Caneparo, Luca;
Concha, Miguel;
Geisler, Robert;
Dale, Trevor;
Wilson, Stephen
All
;
Houart, Corinne;
Take Uchi, Masaya;
Rauch, Gerd;
Young, Neville;
Coutinho, Pedro;
Masai, Ichiro;
Caneparo, Luca;
Concha, Miguel;
Geisler, Robert;
Dale, Trevor;
Wilson, Stephen
All
Abstract
Zebrafish embryos homozygous for the masterblind (mb1) mutation exhibit a striking phenotype in which the eyes and telencephalon are reduced or absent and diencephalic fates expand to the front of the brain. Here we show that mb1(-/-) embryos carry an amino-acid change at a conserved site in the Wnt pathway scaffolding protein, Axin1. The amino-acid substitution present in the mbl allele abolishes the binding of Axin to Gsk3 and affects Tcf-dependent transcription. Therefore, Gsk3 activity may be decreased in mbl(-/-) embryos and in support of this possibility, overexpression of either wild-type Axin1 or Gsk3 beta can restore eye and telencephalic fates to mb1(-/-) embryos. Our data reveal a crucial role for Axin1-dependent inhibition of the Wnt pathway in the early regional subdivision of the anterior neural plate into telencephalic, diencephalic, and eye-forming territories.
Publishing Year
Date Published
2001-06-01
Journal Title
Genes and Development
Volume
15
Issue
11
Page
1427 - 1434
IST-REx-ID
Cite this
Heisenberg C-PJ, Houart C, Take Uchi M, et al. A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon. Genes and Development. 2001;15(11):1427-1434. doi:10.1101/gad.194301
Heisenberg, C.-P. J., Houart, C., Take Uchi, M., Rauch, G., Young, N., Coutinho, P., … Stemple, D. (2001). A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon. Genes and Development. Cold Spring Harbor Laboratory Press. https://doi.org/10.1101/gad.194301
Heisenberg, Carl-Philipp J, Corinne Houart, Masaya Take Uchi, Gerd Rauch, Neville Young, Pedro Coutinho, Ichiro Masai, et al. “A Mutation in the Gsk3-Binding Domain of Zebrafish Masterblind/Axin1 Leads to a Fate Transformation of Telencephalon and Eyes to Diencephalon.” Genes and Development. Cold Spring Harbor Laboratory Press, 2001. https://doi.org/10.1101/gad.194301.
C.-P. J. Heisenberg et al., “A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon,” Genes and Development, vol. 15, no. 11. Cold Spring Harbor Laboratory Press, pp. 1427–1434, 2001.
Heisenberg C-PJ, Houart C, Take Uchi M, Rauch G, Young N, Coutinho P, Masai I, Caneparo L, Concha M, Geisler R, Dale T, Wilson S, Stemple D. 2001. A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon. Genes and Development. 15(11), 1427–1434.
Heisenberg, Carl-Philipp J., et al. “A Mutation in the Gsk3-Binding Domain of Zebrafish Masterblind/Axin1 Leads to a Fate Transformation of Telencephalon and Eyes to Diencephalon.” Genes and Development, vol. 15, no. 11, Cold Spring Harbor Laboratory Press, 2001, pp. 1427–34, doi:10.1101/gad.194301.
Google Scholar