A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon
Heisenberg C-PJ, Houart C, Take Uchi M, Rauch G, Young N, Coutinho P, Masai I, Caneparo L, Concha M, Geisler R, Dale T, Wilson S, Stemple D. 2001. A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon. Genes and Development. 15(11), 1427–1434.
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Journal Article
| Published
| English
Author
Heisenberg, Carl-Philipp ISTA 
;
Houart, Corinne;
Take Uchi, Masaya;
Rauch, Gerd;
Young, Neville;
Coutinho, Pedro;
Masai, Ichiro;
Caneparo, Luca;
Concha, Miguel;
Geisler, Robert;
Dale, Trevor;
Wilson, Stephen
All
;
Houart, Corinne;
Take Uchi, Masaya;
Rauch, Gerd;
Young, Neville;
Coutinho, Pedro;
Masai, Ichiro;
Caneparo, Luca;
Concha, Miguel;
Geisler, Robert;
Dale, Trevor;
Wilson, Stephen
All
Abstract
Zebrafish embryos homozygous for the masterblind (mb1) mutation exhibit a striking phenotype in which the eyes and telencephalon are reduced or absent and diencephalic fates expand to the front of the brain. Here we show that mb1(-/-) embryos carry an amino-acid change at a conserved site in the Wnt pathway scaffolding protein, Axin1. The amino-acid substitution present in the mbl allele abolishes the binding of Axin to Gsk3 and affects Tcf-dependent transcription. Therefore, Gsk3 activity may be decreased in mbl(-/-) embryos and in support of this possibility, overexpression of either wild-type Axin1 or Gsk3 beta can restore eye and telencephalic fates to mb1(-/-) embryos. Our data reveal a crucial role for Axin1-dependent inhibition of the Wnt pathway in the early regional subdivision of the anterior neural plate into telencephalic, diencephalic, and eye-forming territories.
Publishing Year
Date Published
2001-06-01
Journal Title
Genes and Development
Volume
15
Issue
11
Page
1427 - 1434
IST-REx-ID
Cite this
Heisenberg C-PJ, Houart C, Take Uchi M, et al. A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon. Genes and Development. 2001;15(11):1427-1434. doi:10.1101/gad.194301
Heisenberg, C.-P. J., Houart, C., Take Uchi, M., Rauch, G., Young, N., Coutinho, P., … Stemple, D. (2001). A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon. Genes and Development. Cold Spring Harbor Laboratory Press. https://doi.org/10.1101/gad.194301
Heisenberg, Carl-Philipp J, Corinne Houart, Masaya Take Uchi, Gerd Rauch, Neville Young, Pedro Coutinho, Ichiro Masai, et al. “A Mutation in the Gsk3-Binding Domain of Zebrafish Masterblind/Axin1 Leads to a Fate Transformation of Telencephalon and Eyes to Diencephalon.” Genes and Development. Cold Spring Harbor Laboratory Press, 2001. https://doi.org/10.1101/gad.194301.
C.-P. J. Heisenberg et al., “A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon,” Genes and Development, vol. 15, no. 11. Cold Spring Harbor Laboratory Press, pp. 1427–1434, 2001.
Heisenberg C-PJ, Houart C, Take Uchi M, Rauch G, Young N, Coutinho P, Masai I, Caneparo L, Concha M, Geisler R, Dale T, Wilson S, Stemple D. 2001. A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon. Genes and Development. 15(11), 1427–1434.
Heisenberg, Carl-Philipp J., et al. “A Mutation in the Gsk3-Binding Domain of Zebrafish Masterblind/Axin1 Leads to a Fate Transformation of Telencephalon and Eyes to Diencephalon.” Genes and Development, vol. 15, no. 11, Cold Spring Harbor Laboratory Press, 2001, pp. 1427–34, doi:10.1101/gad.194301.
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