Tensile forces govern germ-layer organization in zebrafish

Krieg M, Arboleda Estudillo Y, Puech P, Käfer J, Graner F, Mueller D, Heisenberg C-PJ. 2008. Tensile forces govern germ-layer organization in zebrafish. Nature Cell Biology. 10(4), 429–436.

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Journal Article | Published | English
Author
Krieg, Michael; Arboleda Estudillo, Yohanna; Puech, Pierre; Käfer, Jos; Graner, François; Mueller, Daniel; Heisenberg, Carl-Philipp IST Austria
Abstract
Understanding the factors that direct tissue organization during development is one of the most fundamental goals in developmental biology. Various hypotheses explain cell sorting and tissue organization on the basis of the adhesive and mechanical properties of the constituent cells(1). However, validating these hypotheses has been difficult due to the lack of appropriate tools to measure these parameters. Here we use atomic force microscopy ( AFM) to quantify the adhesive and mechanical properties of individual ectoderm, mesoderm and endoderm progenitor cells from gastrulating zebrafish embryos. Combining these data with tissue self-assembly in vitro and the sorting behaviour of progenitors in vivo, we have shown that differential actomyosin-dependent cell-cortex tension, regulated by Nodal/ TGF beta-signalling ( transforming growth factor beta), constitutes a key factor that directs progenitor-cell sorting. These results demonstrate a previously unrecognized role for Nodal-controlled cell-cortex tension in germ-layer organization during gastrulation.
Publishing Year
Date Published
2008-03-23
Journal Title
Nature Cell Biology
Volume
10
Issue
4
Page
429 - 436
IST-REx-ID

Cite this

Krieg M, Arboleda Estudillo Y, Puech P, et al. Tensile forces govern germ-layer organization in zebrafish. Nature Cell Biology. 2008;10(4):429-436. doi:10.1038/ncb1705
Krieg, M., Arboleda Estudillo, Y., Puech, P., Käfer, J., Graner, F., Mueller, D., & Heisenberg, C.-P. J. (2008). Tensile forces govern germ-layer organization in zebrafish. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb1705
Krieg, Michael, Yohanna Arboleda Estudillo, Pierre Puech, Jos Käfer, François Graner, Daniel Mueller, and Carl-Philipp J Heisenberg. “Tensile Forces Govern Germ-Layer Organization in Zebrafish.” Nature Cell Biology. Nature Publishing Group, 2008. https://doi.org/10.1038/ncb1705.
M. Krieg et al., “Tensile forces govern germ-layer organization in zebrafish,” Nature Cell Biology, vol. 10, no. 4. Nature Publishing Group, pp. 429–436, 2008.
Krieg M, Arboleda Estudillo Y, Puech P, Käfer J, Graner F, Mueller D, Heisenberg C-PJ. 2008. Tensile forces govern germ-layer organization in zebrafish. Nature Cell Biology. 10(4), 429–436.
Krieg, Michael, et al. “Tensile Forces Govern Germ-Layer Organization in Zebrafish.” Nature Cell Biology, vol. 10, no. 4, Nature Publishing Group, 2008, pp. 429–36, doi:10.1038/ncb1705.

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