{"date_created":"2018-12-11T12:07:25Z","publist_id":"1942","doi":"10.1083/jcb.122.2.443","article_processing_charge":"No","date_published":"1993-07-15T00:00:00Z","extern":"1","intvolume":"       122","month":"07","type":"journal_article","day":"15","quality_controlled":"1","date_updated":"2022-03-24T12:59:20Z","oa_version":"None","scopus_import":"1","page":"443 - 450","publication_identifier":{"issn":["0021-9525"]},"year":"1993","publisher":"Rockefeller University Press","_id":"4177","abstract":[{"lang":"eng","text":"Thyroid hormones play an important role in brain development, but the mechanism(s) by which triiodothyronine (T3) mediates neuronal differentiation is poorly understood. Here we demonstrate that T3 regulates the neurotrophic factor, neurotrophin-3 (NT-3), in developing rat cerebellar granule cells both in cell culture and in vivo. In situ hybridization experiments showed that developing Purkinje cells do not express NT-3 mRNA but do express trkC, the putative neuronal receptor for NT-3. Addition of recombinant NT-3 to cerebellar cultures from embryonic rat brain induces hypertrophy and neurite sprouting of Purkinje cells, and upregulates the mRNA encoding the calcium-binding protein, calbindin-28 kD. The present study demonstrates a novel interaction between cerebellar granule neurons and developing Purkinje cells in which NT-3 induced by T3 in the granule cells promotes Purkinje cell differentiation."}],"citation":{"apa":"Lindholm, D., Castrén, E., Tsoulfas, P., Kolbeck, R., Berzaghi, M., Leingärtner, A., … Thoenen, H. (1993). Neurotrophin-3 induced by tri-iodothyronine in cerebellar granule cells promotes Purkinje cell differentiation. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.122.2.443\">https://doi.org/10.1083/jcb.122.2.443</a>","ama":"Lindholm D, Castrén E, Tsoulfas P, et al. Neurotrophin-3 induced by tri-iodothyronine in cerebellar granule cells promotes Purkinje cell differentiation. <i>Journal of Cell Biology</i>. 1993;122(2):443-450. doi:<a href=\"https://doi.org/10.1083/jcb.122.2.443\">10.1083/jcb.122.2.443</a>","short":"D. Lindholm, E. Castrén, P. Tsoulfas, R. Kolbeck, M. Berzaghi, A. Leingärtner, C.-P.J. Heisenberg, L. Tesarollo, L. Parada, H. Thoenen, Journal of Cell Biology 122 (1993) 443–450.","mla":"Lindholm, Dan, et al. “Neurotrophin-3 Induced by Tri-Iodothyronine in Cerebellar Granule Cells Promotes Purkinje Cell Differentiation.” <i>Journal of Cell Biology</i>, vol. 122, no. 2, Rockefeller University Press, 1993, pp. 443–50, doi:<a href=\"https://doi.org/10.1083/jcb.122.2.443\">10.1083/jcb.122.2.443</a>.","chicago":"Lindholm, Dan, Eero Castrén, Pantelis Tsoulfas, Roland Kolbeck, Maria Berzaghi, Axel Leingärtner, Carl-Philipp J Heisenberg, Lino Tesarollo, Luis Parada, and Hans Thoenen. “Neurotrophin-3 Induced by Tri-Iodothyronine in Cerebellar Granule Cells Promotes Purkinje Cell Differentiation.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 1993. <a href=\"https://doi.org/10.1083/jcb.122.2.443\">https://doi.org/10.1083/jcb.122.2.443</a>.","ieee":"D. Lindholm <i>et al.</i>, “Neurotrophin-3 induced by tri-iodothyronine in cerebellar granule cells promotes Purkinje cell differentiation,” <i>Journal of Cell Biology</i>, vol. 122, no. 2. Rockefeller University Press, pp. 443–450, 1993.","ista":"Lindholm D, Castrén E, Tsoulfas P, Kolbeck R, Berzaghi M, Leingärtner A, Heisenberg C-PJ, Tesarollo L, Parada L, Thoenen H. 1993. Neurotrophin-3 induced by tri-iodothyronine in cerebellar granule cells promotes Purkinje cell differentiation. Journal of Cell Biology. 122(2), 443–450."},"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","language":[{"iso":"eng"}],"title":"Neurotrophin-3 induced by tri-iodothyronine in cerebellar granule cells promotes Purkinje cell differentiation","author":[{"first_name":"Dan","last_name":"Lindholm","full_name":"Lindholm, Dan"},{"first_name":"Eero","full_name":"Castrén, Eero","last_name":"Castrén"},{"first_name":"Pantelis","last_name":"Tsoulfas","full_name":"Tsoulfas, Pantelis"},{"first_name":"Roland","last_name":"Kolbeck","full_name":"Kolbeck, Roland"},{"last_name":"Berzaghi","full_name":"Berzaghi, Maria","first_name":"Maria"},{"first_name":"Axel","full_name":"Leingärtner, Axel","last_name":"Leingärtner"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Tesarollo, Lino","last_name":"Tesarollo","first_name":"Lino"},{"first_name":"Luis","last_name":"Parada","full_name":"Parada, Luis"},{"first_name":"Hans","full_name":"Thoenen, Hans","last_name":"Thoenen"}],"publication_status":"published","oa":1,"article_type":"original","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119654/","open_access":"1"}],"issue":"2","acknowledgement":"E. Castrtn is an Alexander von Humboldt fellow. M. Berzaghi is supported by a scholarship from CNPQ, Brasil. L. F. Parada, P. Tsoulfas, and L. Tesarollo were supported by a National Institutes of Health grant. We thank D. Stratmann and K. Angermeyer for skillful technical assistance; I. Hajjar for secretarial work and Dr. R. G~rtner for help with\r\ninducing hypothyroidism; Dr. W. Hunzieker for the calbindin-28 kD eDNA; Dr. M. Fishman for the GAP-43 eDNA; and Dr. Y.-A. Barde for critical comments.","volume":122,"external_id":{"pmid":["8320266"]},"publication":"Journal of Cell Biology","pmid":1,"status":"public"}