TY - JOUR AB - Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells. AU - Riedl, Julia AU - Crevenna, Alvaro H AU - Kessenbrock, Kai AU - Yu, Jerry Haochen AU - Neukirchen, Dorothee AU - Bista, Michal AU - Bradke, Frank AU - Jenne, Dieter AU - Holak, Tad A AU - Werb, Zena AU - Michael Sixt AU - Wedlich-Soldner, Roland ID - 3944 IS - 7 JF - Nature Methods TI - Lifeact: a versatile marker to visualize F-actin VL - 5 ER -