--- res: bibo_abstract: - Cell shape and motility are primarily controlled by cellular mechanics. The attachment of the plasma membrane to the underlying actomyosin cortex has been proposed to be important for cellular processes involving membrane deformation. However, little is known about the actual function of membrane-to-cortex attachment (MCA) in cell protrusion formation and migration, in particular in the context of the developing embryo. Here, we use a multidisciplinary approach to study MCA in zebrafish mesoderm and endoderm (mesendoderm) germ layer progenitor cells, which migrate using a combination of different protrusion types, namely, lamellipodia, filopodia, and blebs, during zebrafish gastrulation. By interfering with the activity of molecules linking the cortex to the membrane and measuring resulting changes in MCA by atomic force microscopy, we show that reducing MCA in mesendoderm progenitors increases the proportion of cellular blebs and reduces the directionality of cell migration. We propose that MCA is a key parameter controlling the relative proportions of different cell protrusion types in mesendoderm progenitors, and thus is key in controlling directed migration during gastrulation.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Alba foaf_name: Diz Muñoz, Alba foaf_surname: Diz Muñoz - foaf_Person: foaf_givenName: Michael foaf_name: Krieg, Michael foaf_surname: Krieg - foaf_Person: foaf_givenName: Martin foaf_name: Bergert, Martin foaf_surname: Bergert - foaf_Person: foaf_givenName: Itziar foaf_name: Ibarlucea Benitez, Itziar foaf_surname: Ibarlucea Benitez - foaf_Person: foaf_givenName: Daniel foaf_name: Müller, Daniel foaf_surname: Müller - foaf_Person: foaf_givenName: Ewa foaf_name: Paluch, Ewa foaf_surname: Paluch - foaf_Person: foaf_givenName: Carl-Philipp J foaf_name: Heisenberg, Carl-Philipp J foaf_surname: Heisenberg foaf_workInfoHomepage: http://www.librecat.org/personId=39427864-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-0912-4566 bibo_doi: 10.1371/journal.pbio.1000544 bibo_issue: '11' bibo_volume: 8 dct_date: 2010^xs_gYear dct_language: eng dct_publisher: Public Library of Science@ dct_title: Control of directed cell migration in vivo by membrane-to-cortex attachment@ ...