Tkacik, GasperISTA ; Gregor, Thomas; Bialek, William S
Gene expression levels fluctuate even under constant external conditions. Much emphasis has usually been placed on the components of this noise that are due to randomness in transcription and translation. Here we focus on the role of noise associated with the inputs to transcriptional regulation; in particular, we analyze the effects of random arrival times and binding of transcription factors to their target sites along the genome. This contribution to the total noise sets a fundamental physical limit to the reliability of genetic control, and has clear signatures, but we show that these are easily obscured by experimental limitations and even by conventional methods for plotting the variance vs. mean expression level. We argue that simple, universal models of noise dominated by transcription and translation are inconsistent with the embedding of gene expression in a network of regulatory interactions. Analysis of recent experiments on transcriptional control in the early Drosophila embryo shows that these results are quantitatively consistent with the predicted signatures of input noise, and we discuss the experiments needed to test the importance of input noise more generally.
NSF Grant PHY-0650617; NIH grants P50 GM071508, R01 GM077599; Burroughs Wellcome Program in Biological Dynamics
Tkačik G, Gregor T, Bialek W. The role of input noise in transcriptional regulation. PLoS One. 2008;3(7). doi:10.1371/journal.pone.0002774
Tkačik, G., Gregor, T., & Bialek, W. (2008). The role of input noise in transcriptional regulation. PLoS One. Public Library of Science. https://doi.org/10.1371/journal.pone.0002774
Tkačik, Gašper, Thomas Gregor, and William Bialek. “The Role of Input Noise in Transcriptional Regulation.” PLoS One. Public Library of Science, 2008. https://doi.org/10.1371/journal.pone.0002774.
G. Tkačik, T. Gregor, and W. Bialek, “The role of input noise in transcriptional regulation,” PLoS One, vol. 3, no. 7. Public Library of Science, 2008.
Tkačik G, Gregor T, Bialek W. 2008. The role of input noise in transcriptional regulation. PLoS One. 3(7).
Tkačik, Gašper, et al. “The Role of Input Noise in Transcriptional Regulation.” PLoS One, vol. 3, no. 7, Public Library of Science, 2008, doi:10.1371/journal.pone.0002774.
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