Accuracy of tetrode spike separation as determined by simultaneous intracellular and extracellular measurements

K. Harris, D. Henze, J.L. Csicsvari, H. Hirase, G. Buzsáki, Journal of Neurophysiology 84 (2000) 401–414.

Download
No fulltext has been uploaded. References only!
Journal Article | Published
Author
; ; ; ;
Abstract
Simultaneous recording from large numbers of neurons is a prerequisite for understanding their cooperative behavior. Various recording techniques and spike separation methods are being used toward this goal. However, the error rates involved in spike separation have not yet been quantified. We studied the separation reliability of “tetrode” (4-wire electrode) recorded spikes by monitoring simultaneously from the same cell intracellularly with a glass pipette and extracellularly with a tetrode. With manual spike sorting, we found a trade-off between Type I and Type II errors, with errors typically ranging from 0 to 30% depending on the amplitude and firing pattern of the cell, the similarity of the waveshapes of neighboring neurons, and the experience of the operator. Performance using only a single wire was markedly lower, indicating the advantages of multiple-site monitoring techniques over single-wire recordings. For tetrode recordings, error rates were increased by burst activity and during periods of cellular synchrony. The lowest possible separation error rates were estimated by a search for the best ellipsoidal cluster shape. Human operator performance was significantly below the estimated optimum. Investigation of error distributions indicated that suboptimal performance was caused by inability of the operators to mark cluster boundaries accurately in a high-dimensional feature space. We therefore hypothesized that automatic spike-sorting algorithms have the potential to significantly lower error rates. Implementation of a semi-automatic classification system confirms this suggestion, reducing errors close to the estimated optimum, in the range 0-8%.
Publishing Year
Date Published
2000-07-01
Journal Title
Journal of Neurophysiology
Volume
84
Issue
1
Page
401 - 414
IST-REx-ID

Cite this

Harris K, Henze D, Csicsvari JL, Hirase H, Buzsáki G. Accuracy of tetrode spike separation as determined by simultaneous intracellular and extracellular measurements. Journal of Neurophysiology. 2000;84(1):401-414.
Harris, K., Henze, D., Csicsvari, J. L., Hirase, H., & Buzsáki, G. (2000). Accuracy of tetrode spike separation as determined by simultaneous intracellular and extracellular measurements. Journal of Neurophysiology, 84(1), 401–414.
Harris, Kenneth, Darrell Henze, Jozsef L Csicsvari, Hajima Hirase, and György Buzsáki. “Accuracy of Tetrode Spike Separation as Determined by Simultaneous Intracellular and Extracellular Measurements.” Journal of Neurophysiology 84, no. 1 (2000): 401–14.
K. Harris, D. Henze, J. L. Csicsvari, H. Hirase, and G. Buzsáki, “Accuracy of tetrode spike separation as determined by simultaneous intracellular and extracellular measurements,” Journal of Neurophysiology, vol. 84, no. 1, pp. 401–414, 2000.
Harris K, Henze D, Csicsvari JL, Hirase H, Buzsáki G. 2000. Accuracy of tetrode spike separation as determined by simultaneous intracellular and extracellular measurements. Journal of Neurophysiology. 84(1), 401–414.
Harris, Kenneth, et al. “Accuracy of Tetrode Spike Separation as Determined by Simultaneous Intracellular and Extracellular Measurements.” Journal of Neurophysiology, vol. 84, no. 1, American Physiological Society, 2000, pp. 401–14.

Export

Marked Publications

Open Data IST Research Explorer

Search this title in

Google Scholar