Associative long-term depression in the hippocampus is dependent on postsynaptic N-type Ca(2+) channels

C. Normann, D. Peckys, C. Schulze, J. Walden, P.M. Jonas, J. Bischofberger, Journal of Neuroscience 20 (2000) 8290–8297.

Download
No fulltext has been uploaded. References only!
Journal Article | Published
Author
; ; ; ; ;
Abstract
Long-term depression (LTD) is a form of synaptic plasticity that can be induced either by low-frequency stimulation of presynaptic fibers or in an associative manner by asynchronous pairing of presynaptic and postsynaptic activity. We investigated the induction mechanisms of associative LTD in CA1 pyramidal neurons of the hippocampus using whole-cell patch-clamp recordings and Ca2+ imaging in acute brain slices. Asynchronous pairing of postsynaptic action potentials with EPSPs evoked with a delay of 20 msec induced a robust, long-lasting depression of the EPSP amplitude to 43%. Unlike LTD induced by low-frequency stimulation, associative LTD was resistant to the application of D-AP-5, indicating that it is independent of NMDA receptors. In contrast, associative LTD was inhibited by (S)-α-methyl-4-carboxyphenyl-glycine, indicating the involvement of metabotropic glutamate receptors. Furthermore, associative LTD is dependent on the activation of voltage-gated Ca2+ channels by postsynaptic action potentials. Both nifedipine, an L-type Ca2+ channel antagonist, and ω-conotoxin GVIA, a selective N-type channel blocker, abolished the induction of associative LTD. 8-hydroxy-2-dipropylaminotetralin (OH-DPAT), a 5-HT(1A) receptor agonist, inhibited postsynaptic Ca2+ influx through N-type Ca2+ channels, without affecting presynaptic transmitter release. OH-DPAT also inhibited the induction of associative LTD, suggesting that the involvement of N-type channels makes synaptic plasticity accessible to modulation by neurotransmitters. Thus, the modulation of N-type Ca2+ channels provides a gain control for synaptic depression in hippocampal pyramidal neurons.
Publishing Year
Date Published
2000-11-15
Journal Title
Journal of Neuroscience
Volume
20
Issue
22
Page
8290 - 8297
IST-REx-ID

Cite this

Normann C, Peckys D, Schulze C, Walden J, Jonas PM, Bischofberger J. Associative long-term depression in the hippocampus is dependent on postsynaptic N-type Ca(2+) channels. Journal of Neuroscience. 2000;20(22):8290-8297.
Normann, C., Peckys, D., Schulze, C., Walden, J., Jonas, P. M., & Bischofberger, J. (2000). Associative long-term depression in the hippocampus is dependent on postsynaptic N-type Ca(2+) channels. Journal of Neuroscience, 20(22), 8290–8297.
Normann, Claus, Diana Peckys, Christian Schulze, Jörg Walden, Peter M Jonas, and Joseph Bischofberger. “Associative Long-Term Depression in the Hippocampus Is Dependent on Postsynaptic N-Type Ca(2+) Channels.” Journal of Neuroscience 20, no. 22 (2000): 8290–97.
C. Normann, D. Peckys, C. Schulze, J. Walden, P. M. Jonas, and J. Bischofberger, “Associative long-term depression in the hippocampus is dependent on postsynaptic N-type Ca(2+) channels,” Journal of Neuroscience, vol. 20, no. 22, pp. 8290–8297, 2000.
Normann C, Peckys D, Schulze C, Walden J, Jonas PM, Bischofberger J. 2000. Associative long-term depression in the hippocampus is dependent on postsynaptic N-type Ca(2+) channels. Journal of Neuroscience. 20(22), 8290–8297.
Normann, Claus, et al. “Associative Long-Term Depression in the Hippocampus Is Dependent on Postsynaptic N-Type Ca(2+) Channels.” Journal of Neuroscience, vol. 20, no. 22, Society for Neuroscience, 2000, pp. 8290–97.

Link(s) to Main File(s)
Access Level
Restricted Closed Access

Export

Marked Publications

Open Data IST Research Explorer

Search this title in

Google Scholar